2026-04-17T20:33:02Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/17612025-10-22T11:25:11Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc López-Cano, Adrià author Bach, Alex author López-Serrano, Sergi author Aragon, Virginia author Blanch, Marta author Pastor, Jose J. author Tedó, Gemma author Morais, Sofia author Garcia-Fruitos, Elena author Alomar, Oscar author 2022-04-21 Antimicrobial resistance is a global threat that is worryingly rising in the livestock sector. Among the proposed strategies, immunostimulant development appears an interesting approach to increase animal resilience at critical production points. The use of nanoparticles based on cytokine aggregates, called inclusion bodies (IBs), has been demonstrated as a new source of immunostimulants in aquaculture. Aiming to go a step further, the objective of this study was to produce cytokine nanoparticles using a food-grade microorganism and to test their applicability to stimulate intestinal mucosa in swine. Four cytokines (IL-1β, IL-6, IL-8, and TNF-α) involved in inflammatory response were produced recombinantly in Lactococcus lactis in the form of protein nanoparticles (IBs). They were able to stimulate inflammatory responses in a porcine enterocyte cell line (IPEC-J2) and alveolar macrophages, maintaining high stability at low pH and high temperature. In addition, an in vivo assay was conducted involving 20 piglets housed individually as a preliminary exploration of the potential effects of IL-1β nanoparticles in piglet intestinal mucosa after a 7 d oral administration. The treated animals tended to have greater levels of TNF-α in the blood, indicating that the tested dose of nanoparticles tended to generate an inflammatory response in the animals. Whether this response is sufficient to increase animal resilience needs further evaluation. López-Cano, Adrià, Alex Bach, Sergi López-Serrano, Virginia Aragon, Marta Blanch, Jose J. Pastor, Gemma Tedó, Sofia Morais, Elena Garcia-Fruitós, and Anna Arís. 2022. "Potential Of Oral Nanoparticles Containing Cytokines As Intestinal Mucosal Immunostimulants In Pigs: A Pilot Study". Animals 12 (9): 1075. doi:10.3390/ani12091075. 2076-2615 http://hdl.handle.net/20.500.12327/1761 https://doi.org/10.3390/ani12091075 Potential of Oral Nanoparticles Containing Cytokines as Intestinal Mucosal Immunostimulants in Pigs: A Pilot Study