2026-04-18T02:22:54Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/1752025-10-22T11:29:31Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Ghebremariam, Michael Kahsay author Hlokwe, Tiny author Rutten, Victor P. M. G. author Allepuz, Alberto author Cadmus, Simeon author Muwonge, Adrian author Robbe-Austerman, Suelee author Michel, Anita L. author 2018-04-17 Mycobacterium bovis (M.bovis) is the main causative agent for bovine tuberculosis (BTB) and can also be the cause of zoonotic tuberculosis in humans. In view of its zoonotic nature, slaughterhouse surveillance, potentially resulting in total or partial condemnation of the carcasses and organs, is conducted routinely. Spoligotyping, VNTR profiling, and whole genome sequencing (WGS) of M. bovis isolated from tissues with tuberculosis-like lesions collected from 14 cattle at Eritrea’s largest slaughterhouse in the capital Asmara, were conducted.The 14 M. bovis isolates were classified into three different spoligotype patterns (SB0120, SB0134 and SB0948) and six VNTR profiles. WGS results matched those of the conventional genotyping methods and further discriminated the six VNTR profiles into 14 strains. Furthermore, phylogenetic analysis of the M. bovis isolates suggests two independent introductions of BTB into Eritrea possibly evolving from a common ancestral strain in Europe.This molecular study revealed the most important strains of M. bovis in Eritrea and their (dis)similarities with the strains generally present in East Africa and Europe, as well as potential routes of introduction of M. bovis. Though the sample size is small, the current study provides important information as well as platform for future in-depth molecular studies on isolates from both the dairy and the traditional livestock sectors in Eritrea and the region. This study provides information onthe origin of some of the M. bovis strains in Eritrea, its genetic diversity, evolution and patterns of spread between dairy herds. Such information is essential in the development and implementation of future BTB control strategy for Eritrea. Ghebremariam, Michael Kahsay, Tiny Hlokwe, Victor P. M. G. Rutten, Alberto Allepuz, Simeon Cadmus, Adrian Muwonge, Suelee Robbe-Austerman, and Anita L. Michel. 2018. "Genetic Profiling Of Mycobacterium Bovis Strains From Slaughtered Cattle In Eritrea". PLOS Neglected Tropical Diseases 12 (4): e0006406. Public Library of Science (PLoS). doi:10.1371/journal.pntd.0006406. 1935-2727 http://hdl.handle.net/20.500.12327/175 https://doi.org/10.1371/journal.pntd.0006406 Genetic profiling of Mycobacterium bovis strains from slaughtered cattle in Eritrea