2026-04-17T13:17:49Zhttps://recercat.cat/oai/request
oai:recercat.cat:20.500.12327/17342025-10-22T11:17:08Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT author Eskova, Anastasia author Chauvigné, Francois author Maischein, Hans-Martin author Ammelburg, Moritz author Cerdà, Joan author Nüsslein-Volhard, Christiane author Irion, Uwe other Producció Animal group Aqüicultura 2025-10-22T11:17:08Z 2025-10-22T11:17:08Z 2017-06-01 Eskova, Anastasia, Francois Chauvigné, Hans-Martin Maischein, Moritz Ammelburg, Joan Cerdà, Christiane Nüsslein-Volhard, and Uwe Irion. 2017. "Gain-Of-Function Mutations Aqp3a Influence Zebrafish Pigment Pattern Formation Through The Tissue Environment". Development. doi:10.1242/dev.143495. 0950-1991 http://hdl.handle.net/20.500.12327/1734 https://doi.org/10.1242/dev.143495 The development of the pigmentation pattern in zebrafish is a tightly regulated process that depends on both the self-organizing properties of pigment cells and extrinsic cues from other tissues. Many of the known mutations that alter the pattern act cell-autonomously in pigment cells, and our knowledge about external regulators is limited. Here, we describe novel zebrafish mau mutants, which encompass several dominant missense mutations in Aquaporin 3a (Aqp3a) that lead to broken stripes and short fins. A loss-of-function aqp3a allele, generated by CRISPR-Cas9, has no phenotypic consequences, demonstrating that Aqp3a is dispensable for normal development. Strikingly, the pigment cells from dominant mau mutants are capable of forming a wild-type pattern when developing in a wild-type environment, but the surrounding tissues in the mutants influence pigment cell behaviour and interfere with the patterning process. The mutated amino acid residues in the dominant alleles line the pore surface of Aqp3a and influence pore permeability. These results demonstrate an important effect of the tissue environment on pigment cell behaviour and, thereby, on pattern formation. eng Attribution 3.0 Unported Gain-of-function mutations in Aqp3a influence zebrafish pigment pattern formation through the tissue environment info:eu-repo/semantics/article