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oai:recercat.cat:20.500.12327/16752025-10-22T11:23:29Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Cantabella, Daniel author Dolcet-Sanjuan, Ramon author Bover-Cid, Sara author Vilanova, Laura author Torres, Rosario author Teixidó, Neus author 2021-09-16 In this study, three wastes based on potato peels and pulps, tomato seeds and wheat bran were used as basis for the preparation of a cheap medium to produce the bacterium P. oryzihabitans PGP01. In flasks experiments, P. oryzihabitans PGP01 growth at 25 °C in a medium based on frozen potato peels and pulp (FPP) with tryptone as a nitrogen source resulted in the maximum production compared to the commercial TSB medium. In the scale-up to 2 L bioreactors, FPP supplemented with tryptone, molasses, NaCl and K2HPO4 allowed to reach similar biomass production than in the TSB medium. A maximum growth of 4.4 × 109 CFU mL−1 after setting the agitation and the air flux conditions at 400 rpm and 0.75 vvm. Finally, P. oryzihabitans PGP01 growing in this optimized medium conserved its biological activity showing the expected effect in root development previously reported for this microorganism. Cantabella, Daniel, Ramon Dolcet-Sanjuan, Cristina Solsona, Laura Vilanova, Rosario Torres, and Neus Teixidó. 2021. "Optimization Of A Food Industry-Waste-Based Medium For The Production Of The Plant Growth Promoting Microorganism Pseudomonas Oryzihabitans PGP01 Based On Agro-Food Industries By-Products". Biotechnology Reports 32: e00675. doi:10.1016/j.btre.2021.e00675. 2215-017X http://hdl.handle.net/20.500.12327/1675 https://doi.org/10.1016/j.btre.2021.e00675 Optimization of a food industry-waste-based medium for the production of the plant growth promoting microorganism Pseudomonas oryzihabitans PGP01 based on agro-food industries by-products