2026-04-17T17:24:33Zhttps://recercat.cat/oai/request
oai:recercat.cat:20.500.12327/16712025-10-22T11:07:43Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT author Aira, Cristina author Klett-Mingo, Jose Ignacio author Ruiz, Tamara author Garcia-Sacristán, Ana author Martín-Valls, Gerard Eduard author Mateu, Enric author Gómez-Laguna, Jaime author Rueda, Paloma author González, Víctor Manuel author Rodríguez, María José author López, Lissette other Producció Animal group Sanitat Animal 2025-10-22T11:07:43Z 2025-10-22T11:07:43Z 2021-08-09 Aira, Cristina, Jose Ignacio Klett-Mingo, Tamara Ruiz, Ana Garcia-Sacristán, Gerard Eduard Martín-Valls, Enric Mateu, and Jaime Gómez-Laguna et al. 2021. "Development Of An Antigen Enzyme-Linked Aptasorbent Assay (ELASA) For The Detection Of Swine Influenza Virus In Field Samples". Analytica Chimica Acta 1181: 338933. doi:10.1016/j.aca.2021.338933. 0003-2670 http://hdl.handle.net/20.500.12327/1671 https://doi.org/10.1016/j.aca.2021.338933 Influenza viruses are highly variable pathogens that infect a wide range of mammalian and avian species. According to the internal conserved proteins (nucleoprotein: NP, and matrix proteins: M), these viruses are classified into type A, B, C, and D. Influenza A virus in swine is of significant importance to the industry since it is responsible for endemic infections that lead to high economic loses derived from poor weight gain, reproductive disorders, and the role it plays in Porcine Respiratory Disease Complex (PRDC). To date, swine influenza virus (SIV) diagnosis continues to be based in complex and expensive technologies such as RT-qPCR. In this study, we aimed to improve actual tools by the implementation of aptamers as capture molecules. First, three different aptamers have been selected using as target the recombinant NP of Influenza A virus expressed in insect cells. Then, these molecules have been used for the development of an Enzyme-Linked AptaSorbent Assay (ELASA) in combination with specific monoclonal antibodies for Influenza A detection. A total of 171 field samples (nasal swabs) have been evaluated with the newly developed assay obtaining a 79.7% and 98.1% sensitivity and specificity respectively, using real time RT-PCR as standard assay. These results suggest that the assay is a promising method that could be used for Influenza A detection in analysis laboratories facilitating surveillance labours. eng Attribution-NonCommercial-NoDerivatives 4.0 International Development of an antigen Enzyme-Linked AptaSorbent Assay (ELASA) for the detection of swine influenza virus in field samples info:eu-repo/semantics/article