2026-04-17T19:06:37Zhttps://recercat.cat/oai/request
oai:recercat.cat:20.500.12327/16342025-10-22T11:04:43Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT author Pérez-Burillo, Javier author Valoti, Greta author Witkowski, Andrezej author Prado, Patricia author Mann, David G. author Trobajo, Rosa other Producció Animal group Aigües Marines i Continentals 2025-10-22T11:04:43Z 2025-10-22T11:04:43Z 2022-01-18 Pérez-Burillo, Javier, Greta Valoti, Andrzej Witkowski, Patricia Prado, David G. Mann, and Rosa Trobajo. 2022. "Assessment Of Marine Benthic Diatom Communities: Insights From A Combined Morphological–Metabarcoding Approach In Mediterranean Shallow Coastal Waters". Marine Pollution Bulletin 174: 113183. doi:10.1016/j.marpolbul.2021.113183. 0025-326X http://hdl.handle.net/20.500.12327/1634 https://doi.org/10.1016/j.marpolbul.2021.113183 We investigated the advantages and disadvantages of light microscope (LM)-based identifications and DNA metabarcoding, based on a 312-bp rbcL marker, for examining benthic diatom communities from Mediterranean shallow coastal environments. For this, we used biofilm samples collected from different substrata in the Ebro delta bays. We show that 1) Ebro delta bays harbour high-diversity diatom communities [LM identified 249 taxa] and 2) DNA metabarcoding effectively reflects this diversity at genus- but not species level, because of the incompleteness of the DNA reference library. Nevertheless, DNA metabarcoding offers new opportunities for detecting small, delicate and rare diatom species missed by LM and diatoms that lack silica frustules. The primers used, though designed for diatoms, successfully amplified rarely reported members of other stramenopile groups. Combining LM and DNA approaches offers stronger support for ecological studies of benthic microalgal communities in shallow coastal environments than using either approach on its own. eng Attribution-NonCommercial-NoDerivatives 4.0 International Assessment of marine benthic diatom communities: insights from a combined morphological–metabarcoding approach in Mediterranean shallow coastal waters info:eu-repo/semantics/article