2026-04-17T18:19:40Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/14792025-10-22T11:10:54Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Parés, Sílvia
author
Cano-Garrido, Olivia
author
Bach, Alex
author
Ferrer-Miralles, Neus
author
Villaverde, Antonio
author
Garcia-Fruitos, Elena
author
Alomar, Oscar
other
Producció Animal
group
Producció de Remugants
2025-10-22T11:10:54Z
2025-10-22T11:10:54Z
2021-11-30
Parés, Sílvia, Olivia Cano-Garrido, Alex Bach, Neus Ferrer-Miralles, Antonio Villaverde, Elena Garcia-Fruitós, and Anna Arís. 2021. "The Potential of Metalloproteinase-9 Administration to Accelerate Mammary Involution and Boost the Immune System at Dry-Off". Animals 11 (12): 3415. doi: 10.3390/ani11123415.
2076-2615
http://hdl.handle.net/20.500.12327/1479
https://doi.org/10.3390/ani11123415
The dry period is decisive for the milking performance of dairy cows. The promptness of
mammary gland involution at dry-off affects not only the productivity in the next lactation, but also
the risk of new intra-mammary infections since it is closely related with the activity of the immune
system. Matrix metalloproteinase-9 (MMP-9) is an enzyme present in the mammary gland and
has an active role during involution by disrupting the extracellular matrix, mediating cell survival
and the recruitment of immune cells. The objective of this study was to determine the potential of
exogenous administration of a soluble and recombinant version of a truncated MMP-9 (rtMMP-9)
to accelerate mammary involution and boost the immune system at dry-off, avoiding the use of
antibiotics. Twelve Holstein cows were dried abruptly, and two quarters of each cow received an
intra-mammary infusion of either soluble rtMMP-9 or a positive control based on immunostimulant
inclusion bodies (IBs). The contralateral quarters were infused with saline solution as negative control.
Samples of mammary secretion were collected during the week following dry-off to determine SCC,
metalloproteinase activity, bovine serum albumin, lactoferrin, sodium, and potassium concentrations.
The soluble form of rtMMP-9 increased endogenous metalloproteinase activity in the mammary
gland compared with saline quarters but did not accelerate either the immune response or involution
in comparison with control quarters. The results demonstrated that the strategy to increase the
mammary gland immunocompetence by recombinant infusion of rtMMP-9 was unsuccessful.
eng
Attribution 4.0 International
The Potential of Metalloproteinase-9 Administration to Accelerate Mammary Involution and Boost the Immune System at Dry-Off
info:eu-repo/semantics/article