2026-04-17T14:51:59Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/14792025-10-22T11:10:54Zcom_2072_4428com_2072_4427col_2072_487898

2025-10-22T11:10:54Z urn:hdl:20.500.12327/1479 The Potential of Metalloproteinase-9 Administration to Accelerate Mammary Involution and Boost the Immune System at Dry-Off Parés, Sílvia Cano-Garrido, Olivia Bach, Alex Ferrer-Miralles, Neus Villaverde, Antonio Garcia-Fruitos, Elena Alomar, Oscar Producció Animal Producció de Remugants The dry period is decisive for the milking performance of dairy cows. The promptness of mammary gland involution at dry-off affects not only the productivity in the next lactation, but also the risk of new intra-mammary infections since it is closely related with the activity of the immune system. Matrix metalloproteinase-9 (MMP-9) is an enzyme present in the mammary gland and has an active role during involution by disrupting the extracellular matrix, mediating cell survival and the recruitment of immune cells. The objective of this study was to determine the potential of exogenous administration of a soluble and recombinant version of a truncated MMP-9 (rtMMP-9) to accelerate mammary involution and boost the immune system at dry-off, avoiding the use of antibiotics. Twelve Holstein cows were dried abruptly, and two quarters of each cow received an intra-mammary infusion of either soluble rtMMP-9 or a positive control based on immunostimulant inclusion bodies (IBs). The contralateral quarters were infused with saline solution as negative control. Samples of mammary secretion were collected during the week following dry-off to determine SCC, metalloproteinase activity, bovine serum albumin, lactoferrin, sodium, and potassium concentrations. The soluble form of rtMMP-9 increased endogenous metalloproteinase activity in the mammary gland compared with saline quarters but did not accelerate either the immune response or involution in comparison with control quarters. The results demonstrated that the strategy to increase the mammary gland immunocompetence by recombinant infusion of rtMMP-9 was unsuccessful. 2025-10-22T11:10:54Z 2025-10-22T11:10:54Z 2021-11-30 info:eu-repo/semantics/article Parés, Sílvia, Olivia Cano-Garrido, Alex Bach, Neus Ferrer-Miralles, Antonio Villaverde, Elena Garcia-Fruitós, and Anna Arís. 2021. "The Potential of Metalloproteinase-9 Administration to Accelerate Mammary Involution and Boost the Immune System at Dry-Off". Animals 11 (12): 3415. doi: 10.3390/ani11123415. 2076-2615 http://hdl.handle.net/20.500.12327/1479 https://doi.org/10.3390/ani11123415 eng Animals MINECO/Programa Nacional de Proyectos de Investigación Fundamental/AGL2012-00028-C02-02/ES/Optimización del periodo de secado de la vaca mediante nanopartículas proteicas/ http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Attribution 4.0 International MDPI