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oai:recercat.cat:20.500.12327/14432025-10-22T11:03:28Zcom_2072_4428com_2072_4427col_2072_487898

Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco Ancín, María Larraya, Luis Florez-Sarasa, Igor Bénard, Camille Fernández-San Millán, Alicia Veramendi, Jon Gibon, Yves Fernie, Alisdair R. Aranjuelo, Iker Farran, Inmaculada Producció Vegetal Genòmica i Biotecnologia 633 In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts. info:eu-repo/semantics/publishedVersion 2021-05-08 info:eu-repo/semantics/article Ancín, María, Luis Larraya, Igor Florez-Sarasa, Camille Bénard, Alicia Fernández-San Millán, Jon Veramendi, Yves Gibon, Alisdair R Fernie, Iker Aranjuelo, and Inmaculada Farran. 2021. "Overexpression Of Thioredoxin M In Chloroplasts Alters Carbon And Nitrogen Partitioning In Tobacco". Journal Of Experimental Botany 72 (13): 4949-4964. doi:10.1093/jxb/erab193. 0022-0957 http://hdl.handle.net/20.500.12327/1443 https://doi.org/10.1093/jxb/erab193 eng Journal of Experimental Botany MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/AGL2016-79868-R/ES/Nuevos criterios de mejora aplicados al incremento en la producción y de calidad de trigo duro en un contesto de cambio climático/ EC/H2020/753301/EU/Exploring the role of the mitochondrial alternative respiration in carotenoid biosynthesis during tomato fruit ripening/ARCATOM MICIU-FEDER/Programa Estatal de promoción del talento y su empleabilidad en I+D+I/RYC2019-027244-I/ES/Metagenomics and integrative biology tools to improve sustainable livestock systems/ Copyright © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess 16 Oxford University Press