2026-04-14T04:20:50Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/14432025-10-22T11:03:28Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Ancín, María
author
Larraya, Luis
author
Florez-Sarasa, Igor
author
Bénard, Camille
author
Fernández-San Millán, Alicia
author
Veramendi, Jon
author
Gibon, Yves
author
Fernie, Alisdair R.
author
Aranjuelo, Iker
author
Farran, Inmaculada
other
Producció Vegetal
group
Genòmica i Biotecnologia
2025-10-22T11:03:28Z
2025-10-22T11:03:28Z
2021-05-08
Ancín, María, Luis Larraya, Igor Florez-Sarasa, Camille Bénard, Alicia Fernández-San Millán, Jon Veramendi, Yves Gibon, Alisdair R Fernie, Iker Aranjuelo, and Inmaculada Farran. 2021. "Overexpression Of Thioredoxin M In Chloroplasts Alters Carbon And Nitrogen Partitioning In Tobacco". Journal Of Experimental Botany 72 (13): 4949-4964. doi:10.1093/jxb/erab193.
0022-0957
http://hdl.handle.net/20.500.12327/1443
https://doi.org/10.1093/jxb/erab193
In plants, there is a complex interaction between carbon (C) and nitrogen (N) metabolism, and its coordination is fundamental for plant growth and development. Here, we studied the influence of thioredoxin (Trx) m on C and N partitioning using tobacco plants overexpressing Trx m from the chloroplast genome. The transgenic plants showed altered metabolism of C (lower leaf starch and soluble sugar accumulation) and N (with higher amounts of amino acids and soluble protein), which pointed to an activation of N metabolism at the expense of carbohydrates. To further delineate the effect of Trx m overexpression, metabolomic and enzymatic analyses were performed on these plants. These results showed an up-regulation of the glutamine synthetase-glutamate synthase pathway; specifically tobacco plants overexpressing Trx m displayed increased activity and stability of glutamine synthetase. Moreover, higher photorespiration and nitrate accumulation were observed in these plants relative to untransformed control plants, indicating that overexpression of Trx m favors the photorespiratory N cycle rather than primary nitrate assimilation. Taken together, our results reveal the importance of Trx m as a molecular mediator of N metabolism in plant chloroplasts.
eng
Copyright © The Author(s) 2021. Published by Oxford University Press on behalf of the Society for Experimental Biology
Overexpression of thioredoxin m in chloroplasts alters carbon and nitrogen partitioning in tobacco
info:eu-repo/semantics/article