2026-04-18T02:10:55Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/12662025-10-22T11:13:46Zcom_2072_4428com_2072_4427col_2072_487898

Transmission of Similar Mcr-1 Carrying Plasmids among Different Escherichia coli Lineages Isolated from Livestock and the Farmer Viñes, Joaquim Cuscó, Anna Napp, Sebastian Alvarez, Julio Saez-Llorente, Jose Luis Rosàs-Rodoreda, Montserrat Francino, Olga Migura-Garcia, Lourdes Producció Animal Sanitat Animal Colistin use has mostly been stopped in human medicine, due to its toxicity. However, nowadays, it still is used as a last-resort antibiotic to treat hospital infections caused by multi-drug resistant Enterobacteriaceae. On the contrary, colistin has been used in veterinary medicine until recently. In this study, 210 fecal samples from pigs (n = 57), calves (n = 152), and the farmer (n = 1) were collected from a farm where E. coli harboring mcr-1-mcr-3 was previously detected. Samples were plated, and mcr-genes presence was confirmed by multiplex-PCR. Hybrid sequencing which determined the presence and location of mcr-1, other antibiotic resistance genes, and virulence factors. Eighteen colistin resistant isolates (13 from calves, four from pigs, and one from the farmer) contained mcr-1 associated with plasmids (IncX4, IncI2, and IncHI2), except for two that yielded mcr-1 in the chromosome. Similar plasmids were distributed in different E. coli lineages. Transmission of mcr-1 to the farmer most likely occurred by horizontal gene transfer from E. coli of calf origin, since plasmids were highly similar (99% coverage, 99.97% identity). Moreover, 33 virulence factors, including stx2 for Shiga toxin E. coli (STEC) were detected, highlighting the role of livestock as a reservoir of pathotypes with zoonotic potential. 2025-10-22T11:13:46Z 2025-10-22T11:13:46Z 2025-10-22T11:13:46Z 2021-03-17 info:eu-repo/semantics/article Viñes, Joaquim, Anna Cuscó, Sebastian Napp, Julio Alvarez, Jose Luis Saez-Llorente, Montserrat Rosàs-Rodoreda, Olga Francino, and Lourdes Migura-Garcia. 2021. "Transmission Of Similar Mcr-1 Carrying Plasmids Among Different Escherichia Coli Lineages Isolated From Livestock And The Farmer". Antibiotics 10 (3): 313. doi:10.3390/antibiotics10030313. 2079-6382 http://hdl.handle.net/20.500.12327/1266 https://doi.org/10.3390/antibiotics10030313 eng Antibiotics MINECO/Programa Estatal de I+D+I orientada a los retos de la sociedad/RTI2018-095586-B-C22/ES/Optimization of diagnosis and treatment of postweaning diarrheas in pigs: towards a rational use of antibiotics/ http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Attribution 4.0 International MDPI