2026-04-18T00:12:56Zhttps://recercat.cat/oai/requestoai:recercat.cat:20.500.12327/12402025-10-22T11:34:11Zcom_2072_4428com_2072_4427col_2072_487898
RECERCAT
author
Carratalá, Jose Vicente
author
Gifre-Renom, Laia
author
Roca-Pinilla, Ramon
author
Villaverde, Antonio
author
Alomar, Oscar
author
Garcia-Fruitos, Elena
author
Sánchez, Julieta María
author
Ferrer-Miralles, Neus
other
Producció Animal
group
Producció de Remugants
2025-10-22T11:34:11Z
2025-10-22T11:34:11Z
2021-03-16
Carratalá, Jose Vicente, Laia Gifre-Renom, Ramon Roca-Pinilla, Antonio Villaverde, Anna Arís, Elena Garcia-Fruitós, Julieta María Sánchez, and Neus Ferrer-Miralles. 2021. "Selecting Subpopulations Of High-Quality Protein Conformers Among Conformational Mixtures Of Recombinant Bovine MMP-9 Solubilized From Inclusion Bodies". International Journal Of Molecular Sciences 22 (6): 3020. doi:10.3390/ijms22063020.
1661-6596
http://hdl.handle.net/20.500.12327/1240
https://doi.org/10.3390/ijms22063020
A detailed workflow to analyze the physicochemical characteristics of mammalian matrix metalloproteinase (MMP-9) protein species obtained from protein aggregates (inclusion bodies—IBs) was followed. MMP-9 was recombinantly produced in the prokaryotic microbial cell factories Clearcoli (an engineered form of Escherichia coli) and Lactococcus lactis, mainly forming part of IBs and partially recovered under non-denaturing conditions. After the purification by affinity chromatography of solubilized MMP-9, four protein peaks were obtained. However, so far, the different conformational protein species forming part of IBs have not been isolated and characterized. Therefore, with the aim to link the physicochemical characteristics of the isolated peaks with their biological activity, we set up a methodological approach that included dynamic light scattering (DLS), circular dichroism (CD), and spectrofluorometric analysis confirming the separation of subpopulations of conformers with specific characteristics. In protein purification procedures, the detailed analysis of the individual physicochemical properties and the biological activity of protein peaks separated by chromatographic techniques is a reliable source of information to select the best-fitted protein populations.
eng
Attribution 4.0 International
Selecting Subpopulations of High-Quality Protein Conformers among Conformational Mixtures of Recombinant Bovine MMP-9 Solubilized from Inclusion Bodies
info:eu-repo/semantics/article