2026-04-14T07:58:35Zhttps://recercat.cat/oai/request

oai:recercat.cat:20.500.12327/12342025-10-22T11:11:26Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Adamo, Irene author Piñuela, Yasmine author Bonet, José Antonio author Castano, Carles author Martínez de Aragón, Juan author Parladé, Javier author Pera, Joan author Alday, Josu G. author 2021-01-23 Soil sampling is a critical step affecting perceived fungal diversity, however sampling optimization for high-throughput-DNA sequencing studies have never been tested in Mediterranean forest ecosystems. We identified the minimum number of pooled samples needed to obtain a reliable description of fungal communities in terms of diversity and composition in three different Mediterranean forests (pine, oak, and mixed-pine-oak). Twenty soil samples were randomly selected in each of the three plots per type. Samples obtained in 100 m2 plots were pooled to obtain mixtures of 3, 6, 10, 15, 20 samples, and sequenced using Illumina MiSeq of fungal ITS2 amplicons. Pooling three soil samples in Pinus and Quercus stands provided consistent richness estimations, while at least six samples were needed in mixed-stands. β-diversity decreased with increasing sample pools in monospecific-stands, while there was no effect of sample pool size on mixed-stands. Soil sample pooling had no effect over species composition. We estimate that three samples would be already optimal to describe fungal richness and composition in Mediterranean pure stands, while at least six samples would be needed in mixed stands. Adamo, Irene, Yasmine Piñuela, José Antonio Bonet, Carles Castaño, Juan Martínez de Aragón, Javier Parladé, Joan Pera, and Josu G. Alday. 2021. "Sampling Forest Soils To Describe Fungal Diversity And Composition. Which Is The Optimal Sampling Size In Mediterranean Pure And Mixed Pine Oak Forests?". Fungal Biology. doi:10.1016/j.funbio.2021.01.005. 1878-6146 http://hdl.handle.net/20.500.12327/1234 https://doi.org/10.1016/j.funbio.2021.01.005 Sampling forest soils to describe fungal diversity and composition. Which is the optimal sampling size in mediterranean pure and mixed pine oak forests?