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oai:recercat.cat:20.500.12327/10662025-10-22T11:25:11Zcom_2072_4428com_2072_4427col_2072_487898

00925njm 22002777a 4500 dc Pérez del Río, Eduardo author Santos, Fabião author Rodriguez Rodriguez, Xavier author Martínez-Miguel, Marc author Roca-Pinilla, Ramon author Alomar, Oscar author Garcia-Fruitos, Elena author Veciana, Jaume author Spatz, Joachim P. author Ratera, Imma author Guasch, Judith author 2020-08-13 Recent achievements in the field of immunotherapy, such as the development of engineered T cells used in adoptive cell therapy, are introducing more efficient strategies to combat cancer. Nevertheless, there are still many limitations. For example, these T cells are challenging to manufacture, manipulate, and control. Specifically, there are limitations in producing the large amounts of therapeutic T cells needed for these therapies in a short period of time and in an economically viable manner. In this study, three-dimensional (3D) poly(ethylene) glycol (PEG) hydrogels covalently combined with low molecular weight heparin are engineered to resemble the lymph nodes, where T cells reproduce. In these hydrogels, PEG provides the needed structural and mechanical properties, whereas heparin is used as an anchor for the cytokine CCL21, which is present in the lymph nodes, and can affect cell migration and proliferation. The 3D structure of the hydrogel in combination with its loading capacity result in an increased primary human CD4+ T cell proliferation compared to the state-of-the-art expansion systems consisting of artificial antigen presenting cells. Thus, we present a new tool for adoptive cell therapy to help achieving the large numbers of cells required for therapy of selected phenotypes targeted against cancer cells, by mimicking the lymph nodes. Pérez del Río, Eduardo, Fabião Santos, Xavier Rodriguez Rodriguez, Marc Martínez-Miguel, Ramon Roca-Pinilla, Anna Arís, and Elena Garcia-Fruitós et al. 2020. "CCL21-Loaded 3D Hydrogels For T Cell Expansion And Differentiation". Biomaterials 259: 120313. doi:10.1016/j.biomaterials.2020.120313. 0142-9612 http://hdl.handle.net/20.500.12327/1066 https://doi.org/10.1016/j.biomaterials.2020.120313 CCL21-loaded 3D hydrogels for T cell expansion and differentiation