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oai:recercat.cat:11351/76162025-10-24T10:37:03Zcom_2072_378070com_2072_378040col_2072_378092

Profiling of Myositis Specific Antibodies and Composite Scores as an Aid in the Differential Diagnosis of Autoimmune Myopathies Mahler, Michael Malyavantham, Kishore Seaman, Andrea Bentow, Chelsea Anunciacion Llunell, Ariadna Sanz Martinez, Maria Teresa Viñas Gimenez, Laura Selva O'Callaghan, Albert Institut Català de la Salut [Mahler M, Malyavantham K, Seaman A, Bentow C] Research and Development, Inova Diagnostics, San Diego, CA, USA. [Anunciacion-Llunell A, Selva-O'Callaghan A] Unitat de Malalties Sistèmiques Autoimmunes, Servei de Medicina Interna, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Sanz-Martínez MT, Viñas-Gimenez L] Servei d’Immunologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain Vall d'Hebron Barcelona Hospital Campus Músculs - Inflamació Autoanticossos Malalties autoimmunitàries DISEASES::Musculoskeletal Diseases::Muscular Diseases::Myositis Other subheadings::Other subheadings::/diagnosis ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Investigative Techniques::Immunologic Techniques::Immunoassay DISEASES::Immune System Diseases::Autoimmune Diseases ENFERMEDADES::enfermedades musculoesqueléticas::enfermedades musculares::miositis Otros calificadores::Otros calificadores::/diagnóstico TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::técnicas inmunológicas::inmunoanálisis ENFERMEDADES::enfermedades del sistema inmune::enfermedades autoinmunes Autoantibodies; Dermatomyositis; Diagnosis Autoanticuerpos; Dermatomiositis; Diagnóstico Autoanticossos; Dermatomiositis; Diagnòstic (1) Background: Myositis specific antibodies (MSA) represent important diagnostic and stratification tools in idiopathic inflammatory myositis (IIM) patients. Here we aimed to evaluate the clinical performance of MSA profiled by a novel particle based multi-analyte technology (PMAT) in IIM and subsets thereof. (2) Methods: 264 IIM patients and 200 controls were tested for MSA using PMAT (Inova Diagnostics, research use only). Diagnostic performance was analyzed and composite scores were generated. (3) Results: The sensitivity/specificity of the individual MSA were: 19.7%/100% (Jo-1), 7.2%/100.0% (Mi-2), 3.0%/99.0% (NXP2), 3.8%/100.0% (SAE), 2.7%/100.0% (PL-7), 1.9%/99.5 (PL-12), 1.1%/100.0% (EJ), 15.5%/99.5% (TIF1γ), 8.3%/98.5% (MDA5), 6.1%/99.0% (HMGCR) and 1.9%/98.5% (SRP). Of all IIM patients, 180/264 tested positive for at least one of the MSAs. In the individual control group, 12/200 (6.0%) tested positive for at least one MSA, most of which had levels close to the cut-off (except one SRP and one PL-12). Only 6/264 (2.3%) IIM patients were positive for more than one antibody (MDA5/HMGCR, EJ/PL-7, 2 x MDA5/TIF1γ, EJ/SAE, SAE/TIF1γ). The overall sensitivity was 68.2% paired with a specificity of 94.0%, leading to an odds ratio of 33.8. The composite scores showed good discrimination between subgroups (e.g., anti-synthetase syndrome). (4) Conclusion: MSA, especially when combined in composite scores (here measured by PMAT), provide value in stratification of patients with IIM. 2022-06-02T08:38:48Z 2022-06-02T08:38:48Z 2021-11-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Mahler M, Malyavantham K, Seaman A, Bentow C, Anunciacion-Llunell A, Sanz-Martínez MT, et al. Profiling of Myositis Specific Antibodies and Composite Scores as an Aid in the Differential Diagnosis of Autoimmune Myopathies. Diagnostics. 2021 Nov 30;11(12):2246. 2075-4418 https://hdl.handle.net/11351/7616 10.3390/diagnostics11122246 34943483 000736636500001 http://hdl.handle.net/11351/7616 eng Diagnostics;11(12) https://doi.org/10.3390/diagnostics11122246 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess application/pdf application/pdf MDPI Scientia