2026-04-17T15:48:31Zhttps://recercat.cat/oai/request

oai:recercat.cat:11351/75702024-12-13T08:59:21Zcom_2072_378071com_2072_378040col_2072_378097

Expression of Chimeric HPV-HIV Protein L1P18 in Pichia pastoris; Purification and Characterization of the Virus-like Particles Eto, Yoshiki Saubí Roca, Narcís Ferrer, Pau Joseph Munné, Joan Institut Català de la Salut [Eto Y] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Departament d’Enginyeria, Química, Biològica i Ambiental, Escola d’Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. AIDS Research Unit, Infectious Diseases Department, Hospital Clínic/IDIBAPS, School of Medicine, University of Barcelona, Barcelona, Spain. [Saubi N] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. AIDS Research Unit, Infectious Diseases Department, Hospital Clínic/IDIBAPS, School of Medicine, University of Barcelona, Barcelona, Spain. [Ferrer P] Departament d’Enginyeria, Química, Biològica i Ambiental, Escola d’Enginyeria, Universitat Autònoma de Barcelona, Bellaterra, Spain. [Joseph-Munné J] Servei de Microbiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain Vall d'Hebron Barcelona Hospital Campus Vacunes Malalties de transmissió sexual - Tractament Papil·lomavirus CHEMICALS AND DRUGS::Complex Mixtures::Biological Products::Vaccines DISEASES::Virus Diseases::Sexually Transmitted Diseases::Sexually Transmitted Diseases, Viral DISEASES::Virus Diseases::DNA Virus Infections::Papillomavirus Infections COMPUESTOS QUÍMICOS Y DROGAS::mezclas complejas::productos biológicos::vacunas ENFERMEDADES::virosis::enfermedades de transmisión sexual::enfermedades virales de transmisión sexual ENFERMEDADES::virosis::infecciones por virus ADN::infecciones por Papillomavirus VPH; Pichia pastoris; Purificación VPH; Pichia pastoris; Purificació HPV; Pichia pastoris; Purification Currently, three human papillomavirus (HPV) vaccines are already licensed and all of them are based on virus-like particles (VLPs) of HPV L1 capsid protein but not worldwide accessible. While about 38.0 million people were living with HIV in 2019, only 68% of HIV-infected individuals were accessing antiretroviral therapy as of the end of June 2020 and there is no HIV vaccine yet. Therefore, safe, effective, and affordable vaccines against those two viruses are immediately needed. Both HPV and HIV are sexually transmitted infections and one of the main access routes is the mucosal genital tract. Thus, the development of a combined vaccine that would protect against HPV and HIV infections is a logical effort in the fight against these two major global pathogens. In this study, a recombinant Pichia pastoris producing chimeric HPV-HIV L1P18 protein intracellularly was constructed. After cell disruption, the supernatant was collected, and the VLPs were purified by a combination of ammonium sulfate precipitation, size exclusion chromatography, ultracentrifugation, and ultrafiltration. At the end of purification process, the chimeric VLPs were recovered with 96% purity and 9.23% overall yield, and the morphology of VLPs were confirmed by transmission electron microscopy. This work contributes towards the development of an alternative platform for production of a bivalent vaccine against HPV and HIV in P. pastoris. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 681137. In addition, we acknowledge support by Instituto de Salud Carlos III (RETIC-RIS RD12/0017, FIS PI14/00494, and FIS PI20/00217), Direcció General de Recerca i Innovació en Salut (DGRIS), Catalan Health Ministry Generalitat de Catalunya, and Centro para el Desarrollo Tecnológico Industrial (CDTI) from the Spanish Ministry of Economy and Business, grant number IDI-20200297. 2022-05-24T06:42:17Z 2022-05-24T06:42:17Z 2021-11-20 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Eto Y, Saubi N, Ferrer P, Joseph-Munné J. Expression of Chimeric HPV-HIV Protein L1P18 in Pichia pastoris; Purification and Characterization of the Virus-like Particles. Pharmaceutics. 2021 Nov 20;13(11):1967. 1999-4923 https://hdl.handle.net/11351/7570 10.3390/pharmaceutics13111967 34834382 000727904600001 http://hdl.handle.net/11351/7570 eng Pharmaceutics;13(11) https://doi.org/10.3390/pharmaceutics13111967 info:eu-repo/grantAgreement/EC/H2020/681137 info:eu-repo/grantAgreement/ES/PE2017-2020/PI20%2F00217 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess application/pdf MDPI Scientia