2026-04-18T03:44:15Zhttps://recercat.cat/oai/request

oai:recercat.cat:11351/74762025-10-24T10:24:46Zcom_2072_378070com_2072_378040col_2072_378092

Analytical Evaluation of Dried Blood Spot and Rapid Diagnostic Test as a New Strategy for Serological Community Screening for Chronic Chagas Disease Silgado Gimenez, Aroa Gual-Gonzalez, Lídia Sánchez Montalvá, Adrián Oliveira Souto, Ines Mercedes Goterris Bonet, Lidia Serre Delcor, Nuria Esperalba Esquerra, Juliana Fernandez Naval, Candela Pumarola Suñé, Tomàs Sulleiro Igual, Elena Gómez i Prat, Jordi Molina Romero, Israel Chagas, Malaltia de - Diagnòstic Sang - Anàlisi Serodiagnòstic DISEASES::Parasitic Diseases::Protozoan Infections::Euglenozoa Infections::Trypanosomiasis::Chagas Disease Other subheadings::Other subheadings::/diagnosis ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Tests, Routine ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES, AND EQUIPMENT::Diagnosis::Diagnostic Techniques and Procedures::Clinical Laboratory Techniques::Clinical Chemistry Tests::Blood Chemical Analysis::Dried Blood Spot Testing ENFERMEDADES::enfermedades parasitarias::infecciones por protozoos::infecciones por Euglenozoa::tripanosomiasis::enfermedad de Chagas Otros calificadores::Otros calificadores::/diagnóstico TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::pruebas diagnósticas rutinarias TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::diagnóstico::técnicas y procedimientos diagnósticos::técnicas de laboratorio clínico::pruebas de bioquímica clínica::análisis químico de la sangre::análisis de manchas de sangre seca Trypanosoma cruzi; Mancha de sangre seca (DBS); Cribado serológico Trypanosoma cruzi; Taca de sang seca (DBS); Cribratge serològic Trypanosoma cruzi; Dried blood spot (DBS); Serological screening Background: Chagas disease is a public health problem not only in Latin America, but also in other regions, including Spain, due to migration movements. Conventional serological diagnosis requires an invasive sample (plasma or serum) and a well-equipped laboratory. To circumvent those limitations, blood samples dried on filter paper (DBS) or Rapid Diagnostic Test (RDT) could be a practical alternative to reference protocol for serological screening in epidemiological studies. We evaluated the usefulness of dried blood sampling and a rapid diagnostic test (Trypanosoma Detect™) for the detection of antibodies against T. cruzi for their use in community-based screening. Methodology/Principal Findings: A total of 162 stored paired whole-blood and serum samples from Latin American migrants and 25 negative-control blood samples were included. Diagnosis of chronic Chagas disease was performed in serum according to WHO algorithms. Blood samples were retrospectively collected as dried spots and then analyzed using two different serological techniques, enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence immunoassay (E-CLIA). Whole-blood samples were also used to evaluate a rapid diagnostic test based on immunochromatography. A better correlation with conventional serum was observed in dried blood elutes using E-CLIA than ELISA (97% vs. 77% sensitivity, respectively). Both assays reported 100% specificity. The median cut-off index values of E-CLIA for dried blood were significantly lower than those for serum (138.1 vs. 243.3, P<0.05). The Trypanosoma Detect™ test presented a sensitivity and specificity of 89.6% and 100%, respectively. Conclusions: The detection of antibodies against T. cruzi in dried blood samples shows a higher sensitivity when using E-CLIA compared with ELISA. Trypanosoma Detect™ is easier to use but has a lower sensitivity. Hence, we propose a sequential strategy based on performing the rapid test first, and a negative result will be confirmed by DBS-ECLIA for use in community Chagas disease screening programs. This work has been supported by the Fundació la Marató TV3 (project number 20182610). 2025-10-24T10:24:46Z 2025-10-24T10:24:46Z 2025-10-24T10:24:46Z 2022-05-04T10:34:10Z 2022-05-04T10:34:10Z 2021-09-15 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/11351/7476 Frontiers in Cellular and Infection Microbiology;11 https://doi.org/10.3389/fcimb.2021.736630 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Frontiers Media Scientia