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oai:recercat.cat:11351/70422024-12-13T09:44:29Zcom_2072_378070com_2072_378040com_2072_378071col_2072_378092col_2072_378097

Angiogenin in the Neurogenic Subventricular Zone After Stroke Gabriel Salazar, Marina Lei (Santiago), Ting Grayston Morales, Alba Costa Riu, Carme Medina Gutierrez, Esperanza Montaner Villalonga, Joan Rosell Novel, Anna Comabella Lopez, Manuel Institut Català de la Salut [Gabriel-Salazar M, Lei T, Grayston A, Medina-Gutiérrez E, Montaner J, Rosell A] Laboratori de Recerca Neurovascular, Servei de Neurologia, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Costa C, Comabella M] Servei de Neurologia-Neuroimmunologia, Centre d'Esclerosi Múltiple de Catalunya (CEMCAT), Barcelona, Spain. Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Vall d’Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain Vall d'Hebron Barcelona Hospital Campus Cèl·lules mare neurals Malalties cerebrovasculars - Tractament ANATOMY::Cells::Stem Cells::Neural Stem Cells DISEASES::Nervous System Diseases::Central Nervous System Diseases::Brain Diseases::Cerebrovascular Disorders::Stroke Other subheadings::Other subheadings::/therapy ANATOMÍA::células::células madre::células madre nerviosas ENFERMEDADES::enfermedades del sistema nervioso::enfermedades del sistema nervioso central::enfermedades cerebrales::trastornos cerebrovasculares::accidente cerebrovascular Otros calificadores::Otros calificadores::/terapia Angiogenina; Exercici; Neuroreparació Angiogenina; Ejercicio; Neuroreparación Angiogenin; Exercise; Neurorepair Ischemic stroke is a leading cause of death and disability worldwide with effective acute thrombolytic treatments. However, brain repair mechanisms related to spontaneous or rehabilitation-induced recovery are still under investigation, and little is known about the molecules involved. The present study examines the potential role of angiogenin (ANG), a known regulator of cell function and metabolism linked to neurological disorders, focusing in the neurogenic subventricular zone (SVZ). Angiogenin expression was examined in the mouse SVZ and in SVZ-derived neural stem cells (NSCs), which were exposed to exogenous ANG treatment during neurosphere formation as well as in other neuron-like cells (SH-SY5Y). Additionally, male C57Bl/6 mice underwent a distal permanent occlusion of the middle cerebral artery to study endogenous and exercise-induced expression of SVZ-ANG and neuroblast migration. Our results show that SVZ areas are rich in ANG, primarily expressed in DCX+ neuroblasts but not in nestin+NSCs. In vitro, treatment with ANG increased the number of SVZ-derived NSCs forming neurospheres but could not modify SH-SY5Y neurite differentiation. Finally, physical exercise rapidly increased the amount of endogenous ANG in the ipsilateral SVZ niche after ischemia, where DCX-migrating cells increased as part of the post-stroke neurogenesis process. Our findings position for the first time ANG in the SVZ during post-stroke recovery, which could be linked to neurogenesis. MG-S was supported by a pre-doctoral fellowship from the Vall d'Hebron Research Institute, AR was supported by the Miguel Servet program and AG holds a pre-doctoral fellowship both from Instituto Carlos III (CPII15/00003 and FI17/00073, respectively), and TL was supported by the China Scholarship Council (CSC-201706170048). This research has been funded by grants PI16/00981, PI19/00186, and RD16/0019/0021 (RETICS-INVICTUS PLUS) from the Instituto de Salud Carlos III co-financed by the European Regional Development Funds, and by the Generalitat de Catalunya (2017SGR 1427). 2022-02-21T09:44:05Z 2022-02-21T09:44:05Z 2021-06-21 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Gabriel-Salazar M, Lei T, Grayston A, Costa C, Medina-Gutiérrez E, Comabella M, et al. Angiogenin in the Neurogenic Subventricular Zone After Stroke. Front Neurol. 2021 Jun 21;12:941. 1664-2295 https://hdl.handle.net/11351/7042 10.3389/fneur.2021.662235 34234733 000669466900001 http://hdl.handle.net/11351/7042 eng Frontiers in Neurology;12 https://doi.org/10.3389/fneur.2021.662235 info:eu-repo/grantAgreement/ES/PE2013-2016/CPII15%2F00003 info:eu-repo/grantAgreement/ES/PE2013-2016/FI17%2F00073 info:eu-repo/grantAgreement/ES/PE2013-2016/PI16%2F00981 info:eu-repo/grantAgreement/ES/PE2017-2020/PI19%2F00186 info:eu-repo/grantAgreement/ES/PE2013-2016/RD16%2F0019%2F0021 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess application/pdf application/pdf Frontiers Media Scientia