2026-04-17T02:51:00Zhttps://recercat.cat/oai/request

oai:recercat.cat:11351/40392025-10-24T10:18:06Zcom_2072_378070com_2072_378040col_2072_378092

Use of IP-10 detection in dried plasma spots for latent tuberculosis infection diagnosis in contacts via mail Villar-Hernández, Raquel Souza Galvao, Maria Luiza de Jiménez Fuentes, Maria Angeles Ruiz-Manzano, J Pilarte Villanueva, Jose Ignacio Cantos Montilla, Adela Altet-Gómez, Neus Latorre, Irene Tuberculosi - Diagnòstic Quimiocines DISEASES::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Positive Bacterial Infections::Actinomycetales Infections::Mycobacterium Infections::Tuberculosis::Latent Tuberculosis Other subheadings::Other subheadings::/diagnosis CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Peptides::Intercellular Signaling Peptides and Proteins::Cytokines::Chemokines::Chemokines, CXC::Chemokine CXCL10 ANALYTICAL, DIAGNOSTIC AND THERAPEUTIC TECHNIQUES AND EQUIPMENT::Investigative Techniques::Epidemiologic Methods::Contact Tracing ENFERMEDADES::infecciones bacterianas y micosis::infecciones bacterianas::infecciones por bacterias grampositivas::infecciones por Actinomycetales::micobacteriosis::tuberculosis::tuberculosis latente Otros calificadores::Otros calificadores::/diagnóstico COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::péptidos::péptidos y proteínas de señalización intercelular::citocinas::quimiocinas::quimiocinas CXC::quimiocina CXCL10 TÉCNICAS Y EQUIPOS ANALÍTICOS, DIAGNÓSTICOS Y TERAPÉUTICOS::técnicas de investigación::métodos epidemiológicos::rastreo de contactos IP-10; Tuberculosis; Mail IP-10; Tuberculosi; Correu IP-10; Tuberculosis; Correo The aim of this study was to test the use of IP-10 detection in dried plasma from contact studies individuals (contacts of smear positive patients), by comparing it with IP-10 and IFN-γ detection in direct plasma, to establish IP-10 detection in DPS as a useful assay for LTBI diagnosis. Whole blood samples were collected from 80 subjects: 12 with active tuberculosis (TB), and 68 from contact studies. The amount of IFN-γ produced by sensitized T cells was determined in direct plasma by QuantiFERON Gold In-Tube test. IP-10 levels were determined in direct and dried plasma by an in-house ELISA. For dried plasma IP-10 determination, two 25 µl plasma drops were dried in Whatman903 filter paper and sent by mail to the laboratory. Regarding TB patients, 100.0%, 91.7% and 75.0% were positive for IFN-γ detection and IP-10 detection in direct and dried plasma, respectively. In contacts, 69.1%, 60.3% and 48.5% had positive results after IFN-γ and IP-10 in direct and dried plasma, respectively. The agreement among in vitro tests was substantial and IP-10 levels in direct and dried plasma were strongly correlated (r = 0.897). In conclusion, IP-10 detection in dried plasma is a simple and safe method that would help improve LTBI management. 2025-10-24T10:18:06Z 2025-10-24T10:18:06Z 2025-10-24T10:18:06Z 2019-04-11T08:49:49Z 2019-04-11T08:49:49Z 2019-03-08 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/11351/4039 Scientific Reports;9(1) https://www.nature.com/articles/s41598-019-40778-1 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ info:eu-repo/semantics/openAccess Nature Research Scientia