2026-04-18T03:59:36Zhttps://recercat.cat/oai/request

oai:recercat.cat:11351/122062025-10-24T10:34:38Zcom_2072_378070com_2072_378040col_2072_378092

00925njm 22002777a 4500 dc Trejo-Zahinos, Jesus author Barberan-Masegosa, Alicia author Bernat, Marta author Perez-Ugarte, Arantxa author Villatoro, Ana Maria author Alcubilla, Pilar author Nadal-Barón, Patricia author Arando, Maider author Sulleiro, Elena author González-López, Juan José author Hoyos Mallecot, Yannick author Antón Pagarolas, Andrés author Larrosa, María Nieves author 2024-11-11T07:31:30Z 2024-11-11T07:31:30Z 2024-10-08 Treponema pallidum; Phylogeny; Syphilis Treponema pallidum; Filogenia; Sífilis Treponema pallidum; Filogènia; Sífilis Worldwide, more than 90% of contemporary syphilis strains belong to SS14-like clade. This study aimed to describe the molecular profile of circulating Treponema pallidum subsp. pallidum (TPA) strains in Barcelona, Spain, from 2021 to 2023 building upon our report in 2015 which showed that 94.8% of typed strains belonged to the SS14 clade. Multilocus sequence typing (MLST) was conducted on TPA-positive samples obtained from swab samples by sequencing the tp0136, tp0548, and tp0705 loci. Strains were classified as Nichols-like or SS14-like clade. Macrolide and tetracycline resistance‑associated mutations were determined through analysis of 23S rDNA and 16S rRNA gene sequences. Of the 96 typeable samples, 47.9% belonged to SS14-like and 52.1% to the Nichols-like. Fourteen haplotypes were identified, with ST26 representing 43.8% of the samples, distributed across 11 haplotypes in the SS14-like and 3 haplotypes in the Nichols-like. All the samples showed macrolide resistance-associated mutations, while none exhibited tetracycline-associated mutations. Our findings revealed a substantial shift in the proportion of TPA clades within the Barcelona population from 2021 to 2023, characterized by a higher proportion of Nichols-like strains compared to 2015 and international trends. The varying temporal and geographical trends underscore the need for regular surveillance to understand regional variations in syphilis and strengthen control programs. http://hdl.handle.net/11351/12206 Anomalies cromosòmiques Sífilis - Epidemiologia Microorganismes - Resistència als medicaments PHENOMENA AND PROCESSES::Genetic Phenomena::Genetic Variation::Mutation ORGANISMS::Bacteria::Bacteria::Spirochaetales::Spirochaetaceae::Treponema::Treponema pallidum DISEASES::Bacterial Infections and Mycoses::Bacterial Infections::Gram-Negative Bacterial Infections::Treponemal Infections::Syphilis PHENOMENA AND PROCESSES::Microbiological Phenomena::Bacterial Physiological Phenomena::Drug Resistance, Bacterial FENÓMENOS Y PROCESOS::fenómenos genéticos::variación genética::mutación ORGANISMOS::Bacteria::Bacteria::Spirochaetales::Spirochaetaceae::Treponema::Treponema pallidum ENFERMEDADES::infecciones bacterianas y micosis::infecciones bacterianas::infecciones por bacterias gramnegativas::infecciones por Treponema::sífilis FENÓMENOS Y PROCESOS::fenómenos microbiológicos::fenómenos fisiológicos bacterianos::farmacorresistencia bacteriana High increase of Nichols-like clade circulating Treponema pallidum subsp. pallidum in Barcelona from 2021 to 2023