2026-04-13T02:57:14Zhttps://recercat.cat/oai/request

oai:recercat.cat:11351/113582025-10-24T10:44:47Zcom_2072_378070com_2072_378040col_2072_378092

Identification of connective tissue disease autoantibodies and a novel autoantibody anti-annexin A11 in patients with “idiopathic” interstitial lung disease Tansley, Sarah McMorrow, Fionnuala Cotton, Caroline V. Adamali, Huzaifa Barratt, Shaney Betteridge, Zoe E. Perurena-Prieto, Janire Autoanticossos Teixit connectiu - Malalties - Diagnòstic Fibrosi pulmonar - Diagnòstic CHEMICALS AND DRUGS::Amino Acids, Peptides, and Proteins::Proteins::Blood Proteins::Immunoproteins::Immunoglobulins::Antibodies::Autoantibodies DISEASES::Skin and Connective Tissue Diseases::Connective Tissue Diseases DISEASES::Respiratory Tract Diseases::Lung Diseases::Lung Diseases, Interstitial::Idiopathic Interstitial Pneumonias::Idiopathic Pulmonary Fibrosis DISEASES::Respiratory Tract Diseases::Lung Diseases::Lung Diseases, Interstitial COMPUESTOS QUÍMICOS Y DROGAS::aminoácidos, péptidos y proteínas::proteínas::proteínas sanguíneas::inmunoproteínas::inmunoglobulinas::anticuerpos::autoanticuerpos ENFERMEDADES::enfermedades de la piel y tejido conjuntivo::enfermedades del tejido conjuntivo ENFERMEDADES::enfermedades respiratorias::enfermedades pulmonares::enfermedades pulmonares intersticiales::neumonías intersticiales idiopáticas::fibrosis pulmonar idiopática ENFERMEDADES::enfermedades respiratorias::enfermedades pulmonares::enfermedades pulmonares intersticiales Autoantibody; Connective tissue disease; Interstitial lung disease Autoanticuerpo; Enfermedad del tejido conectivo; Enfermedad pulmonar intersticial Autoanticòs; Malaltia del teixit connectiu; Malaltia pulmonar intersticial Background Autoantibodies are a hallmark feature of Connective Tissue Diseases (CTD). Their presence in patients with idiopathic interstitial lung disease (ILD) may suggest covert CTD. We aimed to determine the prevalence of CTD autoantibodies in patients diagnosed with idiopathic ILD. Methods 499 patient sera were analysed: 251 idiopathic pulmonary fibrosis (IPF), 206 idiopathic non-specific interstitial pneumonia (iNSIP) and 42 cryptogenic organising pneumonia (COP). Autoantibody status was determined by immunoprecipitation. Results 2.4% of IPF sera had a CTD-autoantibody compared to 10.2% of iNSIP and 7.3% of COP. 45% of autoantibodies were anti-synthetases. A novel autoantibody targeting an unknown 56 kDa protein was found in seven IPF patients (2.8%) and two NSIP (1%) patients. This was characterised as anti-annexin A11. Conclusion Specific guidance on autoantibody testing and interpretation in patients with ILD could improve diagnostic accuracy. Further work is required to determine the clinical significance of anti-annexin A11. This project was supported by a grant from the Liverpool Interstitial Lung Disease Service Charitable Fund. The UK-BILD study has received funding from Arrowe Park Endowment Funds. Funders had no role in study design; in the collection, analysis, and interpretation of data; in the writing of the report; nor in the decision to submit the paper for publication. Research profile myositis line immunoassays were donated by Euroimmun. 2025-10-24T10:44:47Z 2025-10-24T10:44:47Z 2025-10-24T10:44:47Z 2024-04-22T10:29:49Z 2024-04-22T10:29:49Z 2024-05 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/11351/11358 Clinical Immunology;262 https://doi.org/10.1016/j.clim.2024.110201 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Elsevier Scientia