2026-04-17T14:32:54Zhttps://recercat.cat/oai/request

oai:recercat.cat:11351/107182025-10-24T08:28:07Zcom_2072_451660com_2072_378040col_2072_451662

00925njm 22002777a 4500 dc Saez de Gordoa, Karmele author Rodrigo-Calvo, María Teresa author Archilla, Ivan author Lopez-Prades, Sandra author Díaz, Alba author Tarragona, Jordi author Zaffalon, Diana author 2023-12-19T12:07:39Z 2023-12-19T12:07:39Z 2023-11-20 OSNA; Lymph node; Colorectal cancer OSNA; Ganglio linfático; Cáncer colonrectal OSNA; Gangli limfàtic; Càncer colonrectal Early-stage colorectal carcinoma (CRC)-pT1-is a therapeutic challenge and presents some histological features related to lymph node metastasis (LNM). A significant proportion of pT1 CRCs are treated surgically, resulting in a non-negligible surgical-associated mortality rate of 1.5-2%. Among these cases, approximately 6-16% exhibit LNM, but the impact on survival is unclear. Therefore, there is an unmet need to establish an objective and reliable lymph node (LN) staging method to optimise the therapeutic management of pT1 CRC patients and to avoid overtreating or undertreating them. In this multicentre study, 89 patients with pT1 CRC were included. All histological features associated with LNM were evaluated. LNs were assessed using two methods, One-Step Nucleic Acid Amplification (OSNA) and the conventional FFPE plus haematoxylin and eosin (H&E) staining. OSNA is an RT-PCR-based method for amplifying CK19 mRNA. Our aim was to assess the performance of OSNA and H&E in evaluating LNs to identify patients at risk of recurrence and to optimise their clinical management. We observed an 80.9% concordance in LN assessment using the two methods. In 9% of cases, LNs were found to be positive using H&E, and in 24.7% of cases, LNs were found to be positive using OSNA. The OSNA results are provided as the total tumour load (TTL), defined as the total tumour burden present in all the LNs of a surgical specimen. In CRC, a TTL ≥ 6000 CK19 m-RNA copies/µL is associated with poor prognosis. Three patients had TTL > 6000 copies/μL, which was associated with higher tumour budding. The discrepancies observed between the OSNA and H&E results were mostly attributed to tumour allocation bias. We concluded that LN assessment with OSNA enables the identification of pT1 CRC patients at some risk of recurrence and helps to optimise their clinical management. This research was supported by a grant from the Fondo de Investigación Sanitaria PI20/00863 awarded to M.C. and J.C., and PI19/01050 awarded to MP, and it was funded by Instituto de Salud Carlos III and Beca de la Marató de TV3 2020 (Beca la Marato—201932-30) awarded to MP. This research was also co-funded by the European Regional Development Fund/European Social Fund; “A way to make Europe”/“Investing in your future”. CIBEREHD is funded by the Instituto de Salud Carlos III. http://hdl.handle.net/11351/10718 Nodes limfàtics Còlon - Càncer Recte - Càncer ANATOMY::Tissues::Lymphoid Tissue::Lymph Nodes::Sentinel Lymph Node DISEASES::Neoplasms::Neoplasms by Site::Digestive System Neoplasms::Gastrointestinal Neoplasms::Intestinal Neoplasms::Colorectal Neoplasms ANATOMÍA::tejidos::tejido linfoide::ganglios linfáticos::ganglio linfático centinela ENFERMEDADES::neoplasias::neoplasias por localización::neoplasias del sistema digestivo::neoplasias gastrointestinales::neoplasias intestinales::neoplasias colorrectales Lymph Node Molecular Analysis with OSNA Enables the Identification of pT1 CRC Patients at Risk of Recurrence: A Multicentre Study