2026-04-17T14:17:09Zhttps://recercat.cat/oai/request

oai:recercat.cat:10459.1/831312025-02-03T08:01:27Zcom_2072_3622col_2072_479130

Barberry plays an active role as an alternate host of Puccinia graminis in Spain Villegas, Dolors Bartaula, Radhika Cantero-Martínez, Carlos Luster, Douglas Szabo, Les Olivera, Pablo Berlin, Anna Rodriguez-Algaba, Julian Hovmøller, Mogens S. McIntosh, Robert Jin, Yue Aecial host Berberis garciae Berberis hispanica Sexual cycle Stem rust Stem rust, caused by Puccinia graminis, is a destructive group of diseases. The patho - gen uses Berberis species as alternate hosts to complete its life cycle. B. vulgaris and the endemic species B. hispanica and B. garciae are present in Spain. The objective of this study was to investigate the functionality of the indigenous barberry as alternate hosts. Field surveys were conducted in 2018 and 2019 in Huesca, Teruel and Albacete provinces of Spain. Aecial samples on barberry were analysed via infection assays and DNA analysis. B. garciae was predominant in Huesca and Teruel provinces, often found in the field margins of cereal crops. Aecial infections on B. garciae were ob- served in May and uredinial infections on cereal crops in June. Scattered B. hispanica bushes were occasionally found near cereal crops in Albacete, where aecial infections on B. hispanica were observed in June when most cereal crops were mature. Infection assays using aeciospores resulted in stem rust infections on susceptible genotypes of wheat, barley, rye and oat, indicating the presence of the sexual cycle for P. graminis f. sp. tritici, f. sp. secalis and f. sp. avenae. Sequence analyses from aecial samples sup- ported this finding as well as the presence of Puccinia brachypodii. This study provides the first evidence that indigenous Berberis species play an active role in the sexual cycle of P. graminis under natural conditions in Spain The research was supported by the United States Department of Agriculture‐Agricultural Research Service, the Bill and Melinda Gates Foundation and the United Kingdom Department for International Development managed by Cornell University, USA, grant number: DGGW‐OPP1133199, and the State Research Agency (AEI), Spain, project PID2020‐118650RR‐C31. Thanks to Dr Fanny Álvaro for the initial observation and sampling of aecial infection on barberry. The assistance of Dr Joan Pedrol and Dr Rosario Fanlo (University of Lleida, Spain) in grass species identification is gratefully acknowledged. We thank the assistance of Amparo López Olmeda (Teruel Plant Health Service), Gloria Herrero Sánchez (Cereales Teruel Sociedad Cooperativa) and Vicente Lozano Herrera (farmer cereal producer). Meteorological data from the El Ballestero site (Albacete province) were kindly provided by Manuel Fernández. Data from surface cropping area of Aragón have been provided by Sección de estadística del Departamento de Agricultura, Ganadería y Medio Ambiente del Gobierno de Aragón (Silvia Quílez‐Domingo). The support of Daniel Gómez and Alberto Pastoriza (Herbarium curators in the Pyrenean Institute of Ecology, IPE‐CSIC, Jaca) is thankfully acknowledged. Technical assistance of Melissa Carter, Jerry Johnson and Kim‐Phuong Nguyen from USDA‐ARS is acknowledged. Dr Villegas acknowledges the contribution of the CERCA program (Generalitat de Catalunya, Spain). 2022-04-20T09:49:24Z 2022-04-20T09:49:24Z 2022 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://doi.org/10.1111/ppa.13540 0032-0862 http://hdl.handle.net/10459.1/83131 eng Reproducció del document publicat a https://doi.org/10.1111/ppa.13540 Plant pathology, 2022, p.1-11 cc-by (c) Villegas et al., 2022 info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ John Wiley and Sons Inc.