2026-04-18T04:24:29Zhttps://recercat.cat/oai/request

oai:recercat.cat:10459.1/695712024-12-05T21:56:34Zcom_2072_3622col_2072_479130

Identification of a newly conserved SLA-II epitope in a structural protein of swine influenza virus Baratelli, Massimiliano Morgan, Sophie Hemmink, Johanneke Dinie Reid, Elisabeth Carr, Brigid Veronica Lefevre, Eric Montaner Tarbes, Sergio Roberto Charleston, Bryan Fraile Sauce, Lorenzo José Tchilian, Elma Montoya, Maria Swine influenza Class II epitopes Swine Nucleoprotein Despite the role of pigs as a source of new Influenza A Virus viruses (IAV) potentially capable of initiating human pandemics, immune responses to swine influenza virus (SwIV) in pigs are not fully understood. Several SwIV epitopes presented by swine MHC (SLA) class I have been identified using different approaches either in outbred pigs or in Babraham large white inbred pigs, which are 85% identical by genome wide SNP analysis. On the other hand, some class II SLA epitopes were recently described in outbred pigs. In this work, Babraham large white inbred pigs were selected to identify SLA II epitopes from SwIV H1N1. PBMCs were screened for recognition of overlapping peptides covering the NP and M1 proteins from heterologous IAV H1N1 in IFNγ ELISPOT. A novel SLA class II restricted epitope was identified in NP from swine H1N1. This conserved novel epitope could be the base for further vaccine approaches against H1N1 in pigs. The research leading to these results has received funding from the European Community's Seventh Framework Program (FP7, 2007-2013), the Research Infrastructures Action under grant FP7-228393 (a NADIR project); from the project AGL2010-22200-C02-01 from the Spanish Ministry of Science and Innovation; the UK's BBSRC grant BBS/E/I/00002014. 2020-09-30T07:22:29Z 2020-09-30T07:22:29Z 2020 2020-09-30T07:22:29Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://doi.org/10.3389/fimmu.2020.02083 1664-3224 http://hdl.handle.net/10459.1/69571 http://hdl.handle.net/10459.1/69571 eng info:eu-repo/grantAgreement/MICINN//AGL2010-22200-C02-01/ES/CARACTERIZACION DE LA RESPUESTA INMUNE INDUCIDA POR CEPAS DEL VIRUS DE LA GRIPE PORCINA CIRCULANTES EN ESPAÑA. DESARROLLO DE VACUNAS BASADAS EN VLPS QUIMERICAS/ Reproducció del document publicat a: https://doi.org/10.3389/fimmu.2020.02083 Frontiers In Immunology, 2020, vol. 11, p. 2083 info:eu-repo/grantAgreement/EC/FP7/228394 cc-by (c) Baratelli, Massimiliano et al., 2020 info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf Frontiers Media