2026-04-17T14:32:32Zhttps://recercat.cat/oai/request

oai:recercat.cat:10459.1/645182024-12-05T22:27:03Zcom_2072_3622col_2072_479130

00925njm 22002777a 4500 dc Carmona, Andrés author Agüera, Maria L. author Luna-Ruiz, Carlos author Buendía, Paula author Calleros-Basilio, Laura author García-Jerez, Andrea author Rodríguez-Puyol, Manuel author Arias, Manuel author Arias-Guillen, Marta author Arriba, Gabriel de author Ballarín, José author Bernis, Carmen author Fernández i Giráldez, Elvira author García-Rebollo, Sagrario author Mancha, Javier author Peso, Gloria del author Pérez, Estefanía author Poch, Esteban author Portolés, José M. author Rodríguez-Puyol, Diego author Sánchez-Villanueva, Rafael author Sarró, Felipe author Torres, Armando author Martín-Malo, Alejandro author Aljama, Pedro author Ramírez, Rafael author Carracedo, Júlia author 2018-06-15T11:02:28Z 2018-06-15T11:02:28Z 2017-04-01 2018-06-15T11:02:28Z Patients with Stage 5 chronic kidney disease who are on hemodialysis (HD) remain in a chronic inflammatory state, characterized by the accumulation of uremic toxins that induce endothelial damage and cardiovascular disease (CVD). Our aim was to examine microvesicles (MVs), monocyte subpopulations, and angiopoietins (Ang) to identify prognostic markers in HD patients with or without diabetes mellitus (DM). A total of 160 prevalent HD patients from 10 centers across Spain were obtained from the Biobank of the Nephrology Renal Network (Madrid, Spain): 80 patients with DM and 80 patients without DM who were matched for clinical and demographic criteria. MVs from plasma and several monocyte subpopulations (CD142+/CD16+, CD14+/CD162+) were analyzed by flow cytometry, and the plasma concentrations of Ang1 and Ang2 were quantified by ELISA. Data on CVD were gathered over the 5.5 yr after these samples were obtained. MV level, monocyte subpopulations (CD14+/CD162+ and CD142+/CD16+), and Ang2-to-Ang1 ratios increased in HD patients with DM compared with non-DM patients. Moreover, MV level above the median (264 MVs/µl) was associated independently with greater mortality. MVs, monocyte subpopulations, and Ang2-to-Ang1 ratio can be used as predictors for CVD. In addition, MV level has a potential predictive value in the prevention of CVD in HD patients. These parameters undergo more extensive changes in patients with DM. Support for this work was provided by Plan Nacional de IDi Proyectos de Investigación en Salud of Instituto de Salud Carlos III (ISCIII)–Subdirección General de Evaluación, Fondos de desarrollo regional (FEDER; PI11/01536, PI12/01489, PI14/00806, PI15/01785); Junta de Andalucía grants (P010-CTS-6337, P11-CTS-7352); and Fundación Nefrológica. P. Buendía, A. Carmona, and C. Luna-Ruiz are fellows from Consejería de Innovacion, Ciencia y Empresa, Junta de Andalucía. http://hdl.handle.net/10459.1/64518 Chronic kidney disease Cardiovascular disease Diabetes mellitus Markers of endothelial damage in patients with chronic kidney disease on hemodialysis