2026-04-17T12:54:46Zhttps://recercat.cat/oai/request

oai:recercat.cat:10459.1/585152024-12-05T21:23:39Zcom_2072_3622col_2072_479130

2024-12-05T21:23:39Z urn:hdl:10459.1/58515 Engineering complex metabolic pathways in plants Farré Martinez, Gemma Capell Capell, Teresa Zhu, Changfu Christou, Paul Blancquaert, Dieter Van Der Straeten, Dominique Metabolic bottleneck Metabolic branch Metabolic conversion Metabolic diversity Metabolic engineering can be used to modulate endogenous metabolic pathways in plants or introduce new metabolic capabilities in order to increase the production of a desirable compound or reduce the accumulation of an undesirable one. In practice, there are several major challenges that need to be overcome, such as gaining enough knowledge about the endogenous pathways to understand the best intervention points, identifying and sourcing the most suitable metabolic genes, expressing those genes in such a way as to produce a functional enzyme in a heterologous background, and, finally, achieving the accumulation of target compounds without harming the host plant. This article discusses the strategies that have been developed to engineer complex metabolic pathways in plants, focusing on recent technological developments that allow the most significant bottlenecks to be overcome. Research at the Universitat de Lleida is supported by MICINN, Spain (BIO2011-23324, BIO02011-22525, BIO2012-35359, and PIM2010PKB-00746); European Union Framework 7 Program–SmartCell Integrated Project 222716; European Union Framework 7 European Research Council IDEAS Advanced Grant Program-BIOFORCE (to P.C.); RecerCaixa; COST Action FA0804 (Molecular Farming: Plants as a Production Platform for High Value Proteins); and Centre CONSOLIDER on Agrigenomics, funded by MICINN, Spain. Research at Ghent University is supported by Bijzonder Onderzoeksfonds (BOF2004/GOA/012 and BOF2009/GOA/004 to D.V.D.S.) and the Research Foundation Flanders (3G012609 to D.V.D.S.). D.B. is indebted to the Research Foundation Flanders for a PhD fellowship. 2024-12-05T21:23:39Z 2024-12-05T21:23:39Z 2016-11-15T13:22:09Z 2025-01-01 2014 article publishedVersion http://hdl.handle.net/10459.1/58515 info:eu-repo/grantAgreement/MICINN//BIO2011-23324/ES/ESTUDIO DE LA VIABILIDAD DEL AUMENTO DE LA CAPACIDAD FOTOSINTETICA DEL ARROZ SOBREPASANDO LA TRANSICION DE C3 A C4 MEDIANTE INGENIERIA GENETICA/ info:eu-repo/grantAgreement/MICINN//BIO2011-22525/ES/INVESTIGACION DEL MECANISMO DE ACUMULACION DE CAROTENOIDES EN EL ENDOSPERMO DE ARROZ/ info:eu-repo/grantAgreement/MINECO//BIO2012-35359/ES/PRODUCCION DE UNA COMBINACION DE MICROBICIDAS QUE EVITEN LA TRANSMISION DEL VIH EN UNA PLATAFORMA VEGETAL/ info:eu-repo/grantAgreement/MICINN//PIM2010PKB-00746/ES/ Reproducció del document publicat a https://doi.org/10.1146/annurev-arplant-050213-035825 Annual Review of Plant Biology, 2014, vol. 65, p. 187-223 info:eu-repo/grantAgreement/EC/FP7/222716 info:eu-repo/grantAgreement/EC/FP7/232933 (c) Annual Reviews, 2014 info:eu-repo/semantics/restrictedAccess Annual Reviews