2026-04-13T03:09:19Zhttps://recercat.cat/oai/request

oai:recercat.cat:10459.1/571592024-12-05T21:49:04Zcom_2072_3622col_2072_479130

00925njm 22002777a 4500 dc Naqvi, Shaista author Farré Martinez, Gemma author Zhu, Changfu author Sandmann, Gerhard author Capell Capell, Teresa author Christou, Paul author 2016-06-06T10:22:39Z 2025-01-01 2011 The quantity and composition of tocopherols (compounds with vitamin E activity) vary widely among different plant species reflecting the expression, activity and substrate specificity of enzymes in the corresponding metabolic pathway. Two Arabidopsis cDNA clones corresponding to q-hydroxyphenylpyruvate dioxygenase (HPPD) and 2-methyl-6-phytylplastoquinol methyltransferase (MPBQ MT) were constitutively expressed in corn to further characterize the pathway and increase the kernel tocopherol content. Transgenic kernels contained up to 3 times as much c-tocopherol as their wild type counterparts whereas other tocopherol isomers remained undetectable. Biofortification by metabolic engineering offers a sustainable alternative to vitamin E supplementation for the improvement of human health. This study was supported by the Ministerio de Ciencia e Innovación, Spain (BFU2007-61413); BIOFORCE ERC advanced grant to PC; S.N. and G.F. are recipients of Ph.D. fellowships from Ministerio de Ciencia e Innovacio´n, Spain (BES-2005-9161). http://hdl.handle.net/10459.1/57159 Vitamin E Tocopherols Metabolic engineering Transgenic maize Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content