2026-04-13T05:01:26Zhttps://recercat.cat/oai/requestoai:recercat.cat:10459.1/571592024-12-05T21:49:04Zcom_2072_3622col_2072_479130
2024-12-05T21:49:04Z
urn:hdl:10459.1/57159
Simultaneous expression of Arabidopsis ρ-hydroxyphenylpyruvate dioxygenase and MPBQ methyltransferase in transgenic corn kernels triples the tocopherol content
Naqvi, Shaista
Farré Martinez, Gemma
Zhu, Changfu
Sandmann, Gerhard
Capell Capell, Teresa
Christou, Paul
Vitamin E
Tocopherols
Metabolic engineering
Transgenic maize
The quantity and composition of tocopherols
(compounds with vitamin E activity) vary widely
among different plant species reflecting the expression,
activity and substrate specificity of enzymes in the
corresponding metabolic pathway. Two Arabidopsis
cDNA clones corresponding to q-hydroxyphenylpyruvate
dioxygenase (HPPD) and 2-methyl-6-phytylplastoquinol
methyltransferase (MPBQ MT) were
constitutively expressed in corn to further characterize
the pathway and increase the kernel tocopherol content.
Transgenic kernels contained up to 3 times as
much c-tocopherol as their wild type counterparts
whereas other tocopherol isomers remained undetectable.
Biofortification by metabolic engineering offers a
sustainable alternative to vitamin E supplementation
for the improvement of human health.
This study was supported by the Ministerio de Ciencia e Innovación, Spain (BFU2007-61413); BIOFORCE ERC advanced grant to PC; S.N. and G.F. are recipients of Ph.D. fellowships from Ministerio de Ciencia e Innovacio´n, Spain (BES-2005-9161).
2024-12-05T21:49:04Z
2024-12-05T21:49:04Z
2016-06-06T10:22:39Z
2025-01-01
2011
article
publishedVersion
http://hdl.handle.net/10459.1/57159
info:eu-repo/grantAgreement/MEC//BFU2007-61413/ES/
Reproducció del document publicat a https://doi.org/10.1007/s11248-010-9393-6
Transgenic Research, 2011, vol. 20, núm. 1, p. 177-181
info:eu-repo/grantAgreement/EC/FP7/232933
(c) Springer Science+Business Media B.V., 2010
info:eu-repo/semantics/restrictedAccess
Springer Verlag