2026-04-17T02:58:38Zhttps://recercat.cat/oai/request

oai:recercat.cat:10459.1/493472024-12-05T21:48:48Zcom_2072_3622col_2072_479130

00925njm 22002777a 4500 dc Belanche, A. author Abecia, Leticia author Holtrop, G. author Guada, J. A. author Castrillo, C. author de la Fuente Oliver, Gabriel author Balcells Terés, Joaquim author 2016-01-19T18:47:00Z 2016-01-19T18:47:00Z 2011-06-29 2016-01-19T18:46:03Z The aim of this study was to investigate the effect of presence or absence of protozoa on rumen fermentation and efficiency of microbial protein synthesis under different diets. Of 20 twin paired lambs, 1 lamb of each pair was isolated from the ewe within 24 h after birth and reared in a protozoa-free environment (n = 10), whereas their respective twin-siblings remained with the ewe (faunated, n = 10). When lambs reached 6 mo of age, 5 animals of each group were randomly allocated to 1 of 2 experimental diets consisting of either alfalfa hay as the sole diet, or 50:50 mixed with ground barley grain according to a 2 × 2 factorial arrangement of treatments. After 15 d of adaptation to the diet, the animals were euthanized and total rumen and abomasal contents were sampled to estimate rumen microbial synthesis using C31 alkane as flow marker. Different (15N and purine bases) and a novel (recombinant DNA sequences) microbial markers, combined with several microbial reference extracts (rumen protozoa, liquid and solid associated bacteria) were evaluated. Absence of rumen protozoa modified the rumen fermentation pattern and decreased total tract OM and NDF digestibility in 2.0 and 5.1 percentage points, respectively. The effect of defaunation on microbial N flow was weak, however, and was dependent on the microbial marker and microbial reference extract considered. Faunated lambs fed with mixed diet showed the greatest rumen protozoal concentration and the least efficient microbial protein synthesis (29% less than the other treatments), whereas protozoa-free lambs fed with mixed diet presented the smallest ammonia concentration and 34% greater efficiency of N utilization than the other treatments. Although 15N gave the most precise estimates of microbial synthesis, the use of recombinant DNA sequences represents an alternative that allows separate quantification of the bacteria and protozoa contributions. This marker showed that presence of protozoa decrease the bacterial-N flow through the abomasum by 33%, whereas the protozoa-N contribution to the microbial N flow increased from 1.9 to 14.1% when barley grain was added to the alfalfa hay. Absolute data related to intestinal flow must be treated with caution because the limitations of the sampling and maker system employed. This work was supported by CICYT project AGL 2004-02910/GAN and an FPU grant awarded to the first author (A. Belanche) by the Spanish Ministry of Education and Science. Holtrop was funded by a core grant to Biomathematics and Statistics Scotland from the Scottish Executive Rural and Environment Research and Analysis Directorate. Thanks are due Maria del Carmen García and the staff from the animal research service from the University of Zaragoza (Zaragoza, Spain). http://hdl.handle.net/10459.1/49347 Remugants Protozous Purins Ruminants Protozoa Liquid farm manure Study of the effect of presence or absence of protozoa on rumen fermentation and microbial protein contribution to the chyme