2026-04-13T03:54:58Zhttps://recercat.cat/oai/request

oai:recercat.cat:10459.1/493462024-12-05T22:01:05Zcom_2072_3622col_2072_479130

00925njm 22002777a 4500 dc Belanche, A. author de la Fuente Oliver, Gabriel author Yáñez-Ruiz, D. R. author Newbold, C. Jamie author Calleja, L. author Balcells Terés, Joaquim author 2016-01-19T18:29:12Z 2016-01-19T18:29:12Z 2011-04-12 2016-01-19T18:17:57Z Two groups of 5 lambs were euthanized at the weaning (T45) and fattening stages (T90) to evaluate the use of microbial ribosomal DNA (rDNA) sequences as potential microbial markers in relation to purine bases (PB) as a conventional marker. Both microbial markers originated similar microbial N concentrations (mg/g of DM), although T45 showed decreased values compared with the T90 group when either PB or rDNA were considered (P = 0.02). The survival of microbial rDNA was determined in 3 digestive sites (omasum, abomasum, and duodenum), but no substantial differences were observed, indicating that rDNA maintains the molecular stability along the sampling sites analyzed. Contrarily PB concentration increased successively along the digestive tract (P < 0.05), likely as a consequence of the endogenous PB secretion. Undegraded milk PB may also explain the overestimation of the microbial N concentration (2.8 times greater) using PB than rDNA sequences. Abomasum was the sampling site where the best agreement between PB and rDNA estimations was observed. Protozoal N concentration was irrelevant in T45 animals, although substantial in T90 lambs (18% of microbial N). In conclusion, bacterial 16S and protozoal 18S rDNA sequences may persist through the gastric digestive tract and their utilization as a highly specific microbial marker should not be neglected. This study was supported by a FPU grant from the Education and Science Spanish Ministry (project: AGL 2004-02910/GAN) and by a University of Zaragoza project (UZ2008-BIO-04). http://hdl.handle.net/10459.1/49346 Bestiar oví Bacteris Protozous Purins Sheep Bacteria Protozoa Liquid farm manure Technical note: The persistence of microbial-specific DNA sequences through gastric digestion in lambs and their potential use as microbial markers