2026-04-17T02:25:32Zhttps://recercat.cat/oai/requestoai:recercat.cat:10459.1/492222024-12-05T21:48:08Zcom_2072_3622col_2072_479130
RECERCAT
author
de la Fuente Oliver, Gabriel
author
Cebrián, J. A.
author
Fondevila, Manuel
2024-12-05T21:48:08Z
2024-12-05T21:48:08Z
2015-12-02T17:25:32Z2025-01-0120042015-12-02T17:25:32Z
http://hdl.handle.net/10459.1/49222
Aims: To study the viability of a culture of the rumen protozoon Entodinium caudatum after a cryopreservation procedure by a fluorescence microscopy staining method. Methods and Results: Fluorescence method is based on the different colour of cells depending on their membrane integrity. When the temperature effect was studied either by fluorescence or motility, the techniques were correlated (r = 0·727) and their slopes and intercepts were not different (P > 0·05). However, motility showed a higher variation coefficient (0·40 vs 0·12). There were no differences between cooling rates at cryopreservation (1 and 4°C min−1) at 38, 15 or 5°C, nor after thawing. Conclusions: Fluorescence staining is more accurate than motility for assessing protozoal viability. Viability after thawing was 0·50, and the number of viable cells per 250 μl straw was 320 and 420 for 1 and 4°C min−1. Significance and Impact of the Study: This cryopreservation procedure seems to ensure culture recovery for E. caudatum.This work has been financed by the project AGL2004-02910. Thanks are given to Prof B.A. Dehority for hiscritical review of the manuscript.
(c) Blackwell Science, 2004 info:eu-repo/semantics/restrictedAccess
Remugants
Protozous
Criobiologia
Ruminants
Protozoa
Cryobiology
A cryopreservation procedure for the rumen protozoon Entodinium caudatum: estimation of its viability by fluorescence microscopy
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion