2026-04-13T06:38:22Zhttps://recercat.cat/oai/request

oai:recercat.cat:10459.1/492222024-12-05T21:48:08Zcom_2072_3622col_2072_479130

00925njm 22002777a 4500 dc de la Fuente Oliver, Gabriel author Cebrián, J. A. author Fondevila, Manuel author 2015-12-02T17:25:32Z 2025-01-01 2004 2015-12-02T17:25:32Z Aims:  To study the viability of a culture of the rumen protozoon Entodinium caudatum after a cryopreservation procedure by a fluorescence microscopy staining method. Methods and Results:  Fluorescence method is based on the different colour of cells depending on their membrane integrity. When the temperature effect was studied either by fluorescence or motility, the techniques were correlated (r = 0·727) and their slopes and intercepts were not different (P > 0·05). However, motility showed a higher variation coefficient (0·40 vs 0·12). There were no differences between cooling rates at cryopreservation (1 and 4°C min−1) at 38, 15 or 5°C, nor after thawing. Conclusions:  Fluorescence staining is more accurate than motility for assessing protozoal viability. Viability after thawing was 0·50, and the number of viable cells per 250 μl straw was 320 and 420 for 1 and 4°C min−1. Significance and Impact of the Study:  This cryopreservation procedure seems to ensure culture recovery for E. caudatum. This work has been financed by the project AGL2004-02910. Thanks are given to Prof B.A. Dehority for hiscritical review of the manuscript. http://hdl.handle.net/10459.1/49222 Remugants Protozous Criobiologia Ruminants Protozoa Cryobiology A cryopreservation procedure for the rumen protozoon Entodinium caudatum: estimation of its viability by fluorescence microscopy