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oai:recercat.cat:10459.1/4651672025-09-15T18:56:38Zcom_2072_3622col_2072_479130

Tailoring the techno-functional properties of fava bean protein isolate: A comparative evaluation of ultrasonication and pulsed electric field treatments Gulzar, Saqib Martín Belloso, Olga Soliva-Fortuny, Robert Ultrasonication Pulsed electric fields Fava bean protein isolate Protein functionality Plant-based proteins The fava bean protein isolate (FBPI) holds promise as a sustainable plant-based protein ingredient. However, native FBPIs exhibit limited functionality, including unsuitable emulsifying activities and a low solubility at a neutral pH, restricting their applications. This study is focused on the effect of ultrasonication (US) and pulsed electric fields (PEF) on modulating the techno-functional properties of FBPIs. Native FBPIs were treated with US at amplitudes of 60–90% for 30 min in 0.5 s on-and-off cycles and with PEF at an electric field intensity of 1.5 kV/cm with 1000–4000 pulses of 20 μs pulse widths. US caused a reduction in the size and charge of the FBPIs more prominently than the PEF. Protein characterization by means of SDS-PAGE illustrated that US and PEF caused severe-to-moderate changes in the molecular weight of the FBPIs. In addition, a spectroscopic analysis using Fourier-transform infrared (FTIR) and circular dichroism (CD) revealed that US and the PEF induced conformational changes through partial unfolding and secondary structure remodeling from an α-helix to a β-sheet. Crystallographic and calorimetric determinations indicated decreased crystallinity and lowered thermal transition temperatures of the US- and PEF-modified FBPIs. Overall, non-thermal processing provided an effective strategy for upgrading FBPIs’ functionality, with implications for developing competitive plant-based protein alternatives. This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 101034288. 2024 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Reproducció del document publicat a https://doi.org/10.3390/foods13030376 Foods, 2024, vol. 13, núm. 376, p.1-20 info:eu-repo/grantAgreement/EC/H2020/101034288/EU/IberusExperience cc by (c) Gulzar et al., 2024 Attribution 4.0 International info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ MDPI