2026-04-17T04:32:03Zhttps://recercat.cat/oai/request
oai:recercat.cat:10459.1/4649432025-09-15T18:37:24Zcom_2072_3622col_2072_479130
RECERCAT author Yeramian Hakim, Andree author Sorolla Bardají, Anabel author Velasco Sánchez, Ana author Santacana Espasa, Maria author Dolcet Roca, Xavier author Valls Marsal, Joan author Abal Diaz, Leandro author Moreno, Sara author Egido Garcia, Ramon Maria author Casanova i Seuma, Josep M. (Josep Manel) author Puig, Susana author Vilella, Ramón author Llombart, Antonio author Matias-Guiu, Xavier author Martí Laborda, Rosa Ma. 2012 Despite the use of multiple therapeutic strategies, metastatic melanoma remains a challenge for oncologists. Thus, new approaches using combinational treatment may be used to try to improve the prognosis of this disease. In this report, we have analyzed the expression of receptor tyrosine kinases (RTKs) in melanoma specimens and in four metastatic melanoma cell lines. Both melanoma specimens and cell lines expressed RTKs, suggesting that they may represent eventual targets for multitargeted tyrosine kinase inhibitor, Suntinib. Sunitinib reduced the proliferation of two melanoma cell lines (M16 and M17) and increased apoptosis in one of them (M16). Moreover, the two metastatic melanoma cell lines harbored an activated receptor (PDGFRα and VEGFR, respectively), and Sunitinib suppressed the phosphorylation of the RTKs and their downstream targets Akt and ribosomal protein S6, in these two cell lines. Similar results were obtained when either PDGFRα or VEGFR2 expression was silenced by lentiviral-mediated short-hairpin RNA delivery in M16 and M17, respectively. To evaluate the interaction between Sunitinib and Bortezomib, median dose effect analysis using MTT assay was performed, and combination index was calculated. Bortezomib synergistically enhanced the Sunitinib-induced growth arrest in Sunitinib-sensitive cells (combination index < 1). Moreover, LY294002, a PI3K inhibitor, sensitized melanoma cells to Bortezomib treatment, suggesting that downregulation of phospho-Akt by Sunitinib mediates the synergy obtained by Bortezomib + Sunitinib cotreatment. Altogether, our results suggest that melanoma cells harboring an activated RTK may be clinically responsive to pharmacologic RTK inhibition by Sunitinib, and a strategy combining Sunitinib and Bortezomib, may provide therapeutic benefit. (C) UICC, 2011 info:eu-repo/semantics/openAccess Melanoma Bortezomib RTK Sunitinib Akt Survival Apoptosis Inhibition of activated receptor tyrosine kinases by Sunitinib induces growth arrest and sensitizes melanoma cells to Bortezomib by blocking Akt pathway info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion