2026-04-14T04:28:23Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/274412025-12-05T14:05:16Zcom_2072_452955com_2072_2054col_2072_452958

The Oxytocin Receptor in Spermatozoa May Originate From Both Spermatogenesis and Epididymal Maturation, and Regulates Capacitation Martínez-Hernández, Jesús Garriga, Ferran Ahmad, Adeel Padilla, Lorena Maside, Carolina Bonet, Sergi Barranco, Isabel Roca, Jordi Pastor, Luis Miguel Yeste Oliveras, Marc Oxitocina Oxytocin Porcs -- Espermatozoides Swine -- Spermatozoa Espermatogènesi en els animals Spermatogenesis in animals Fecundació in vitro Fertilization in vitro Background: The oxytocin receptor (OR) is a G-protein-coupled receptor recently identified in human spermatozoa, whose origin and role in sperm physiology remain unknown. Objectives: In this study, using the pig as a model, we examine the presence of the OR in ejaculated spermatozoa through immunofluorescence and immunoblotting, and investigate the receptor's origin in the male gamete via immunohistochemistry in testicular and epididymal tissues. Additionally, we assess the involvement of the OR in in vitro capacitation and the acrosome reaction by utilizing physiological concentrations of agonists (oxytocin and carbetocin) and an antagonist (L-371,257). Results: The results indicate that, in addition to the expected presence in ejaculated spermatozoa, the OR is expressed during spermatogenesis. Besides, this receptor is found in Leydig and Sertoli cells, as well as in the principal, basal, and apical cells of the epididymis. Furthermore, our data suggest that, during epididymal maturation, the OR could be incorporated in spermatozoa via extracellular vesicles within the apical blebs. The OR is involved in sperm capacitation, as the combination of the antagonist (L-371,257) and the agonist (carbetocin) increases intracellular calcium levels and membrane lipid disorders, which are known as capacitation markers. Conclusions: The presence of the OR in mammalian spermatozoa could originate from both spermatogenesis and epididymal maturation. Moreover, in the male gamete, this receptor regulates sperm capacitation by interacting with its ligand in the female reproductive tract Research funding: European Union-Next Generation EU Funds and University of Murcia. Grant Number: 181/MSJD/22; Ministry of Science, Innovation and Universities. Grant Numbers: PID2020-113320RB-I00, PRE2021-098896; Catalan Institution for Research and Advanced Studies. Grant Number: ICREA-Academia and Agency for Management of University and Research Grants. Grant Number: 2021-SGR-00900 Open Access funding provided thanks to the CRUE-CSIC agreement with Wiley 2025-09 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed info:eu-repo/semantics/altIdentifier/doi/10.1111/andr.70123 info:eu-repo/semantics/altIdentifier/issn/2047-2919 info:eu-repo/semantics/altIdentifier/eissn/2047-2927 PID2020-113320RB-I00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113320RB-I00/ES/IMPLICACION DE LA FUNCION MITOCONDRIAL EN LA LONGEVIDAD DEL ESPERMATOZOIDE DE ESPECIES DE INTERES PRODUCTIVO Y DISEÑO DE DILUYENTES PARA SU CONSERVACION/ Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess Wiley Andrology, 2025, vol. undef, núm. undef, p. undef Articles publicats (D-B)