2026-04-13T00:54:57Zhttps://recercat.cat/oai/requestoai:recercat.cat:10256/265132025-03-05T20:29:33Zcom_2072_452955com_2072_2054col_2072_452960
RECERCAT
author
Kühne, Britta A.
author
Teixidó, Elisabet
author
Ettcheto, Miren
author
Puig i Miquel, Teresa
author
Planas i Grabuleda, Marta
author
Feliu Soley, Lidia
author
Pla, Laura
author
Campuzano, Victoria
author
Gratacós, Eduard
author
Fritsche, Ellen
author
Illa, Miriam
author
Barenys, Marta
2025-03-05T20:29:33Z
2025-03-05T20:29:33Z
2022-10-29
http://hdl.handle.net/10256/26513
Following a multi-disciplinary approach integrating information from several experimental models we have collected new evidence supporting, expanding and redesigning the AOP 'Disrupted laminin/int-β1 interaction leading to decreased cognitive function'. Investigations in vitro in rabbit and rat neurospheres and in vivo in mice exposed to EGCG (epigallocatechin-gallate) during neurodevelopment are combined with in vitro evaluations in neural progenitor cells overexpressing int-β1 and literature information from int-β1 deficiency models. We have discovered for the first time that neural progenitor cells from intrauterine growth restricted (IUGR) animals overexpress int-β1 at gene and protein level and due to this change in prenatal brain programming they respond differently than control neurospheres to the exposure of EGCG, a compound triggering neural progenitor cell migration alterations. We have also identified that EGCG developmental exposure has deleterious effects on neuronal branching and arborization in vitro and in vivo. Our results warn that a thorough developmental neurotoxicity characterization of this and other catechin-based food supplements is needed before recommending their consumption during pregnancyThis study has been funded by Instituto de Salud Carlos III through the project “PI18/01763” (co-funded by European Regional Development Fund, "A way to make Europe"), from “LaCaixa” Foundation under grant agreements LCF/PR/GN14/10270005 and LCF/PR/GN18/10310003, and from AGAUR under grant 2017 SGR n° 1531. B.A.K. received a scholarship from Fundació Bosch i Gimpera (project number: 300155)
Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess
Neurotoxicologia
Neurogenètica
Cèl·lules mare embrionàries
Neurotoxicology
Neurogenetics
Embryonic stem cells
Application of the adverse outcome pathway to identify molecular changes in prenatal brain programming induced by IUGR: Discoveries after EGCG exposure
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed