2026-04-04T01:28:11Zhttps://recercat.cat/oai/request
oai:recercat.cat:10256/259812025-01-16T00:37:46Zcom_2072_452955com_2072_2054col_2072_452958
RECERCAT author Yeste Oliveras, Marc author Briz González, Maria Dolors author Pinart Nadal, Elisabeth author Sancho Badell, Sílvia author Garcia Gil, Núria author Badia Brea, Maria Elena author Bassols Casadevall, Judit author Pruneda Sais, Anna author Bussalleu Muntada, Eva author Casas Roqueta, Isabel author Bonet, Sergi 2025-01-16T00:37:45Z 2025-01-16T00:37:45Z 2008-10 http://hdl.handle.net/10256/25981 Prostaglandin F2α (PGF2α) has been used to improve reproductive performance in swine. The goal of the present work was to determine how the addition of PGF2α affects boar sperm quality. Eleven different treatments were evaluated: eight with only PGF2α (0.625, 1.25, 2.50, 5, 10, 12.50, 25 and 50 mg PGF2α/100 ml) and three binary treatments (0.625 mg PGF2α/100 ml + 200 μg/ml hyaluronic acid (HA), 1.25 mg PGF2α/100 ml + 200 μg/ml HA, 0.625 mg PGF2α/100 ml + 7.5 μM caffeine (Caf)). All these substances were added to 16 ejaculates from 16 healthy and sexually mature boars (n = 16), and each ejaculate was considered as a replicate. Our study also assessed the effects of these 11 treatments over different periods of preservation. Sperm quality was tested immediately after the addition of treatments (time 0), and after 1, 3, 6 and 10 days of cooling at 15 °C. To evaluate sperm quality, five parameters were analysed: (1) sperm viability, acrosome and mitochondrial sheath integrity (using a multiple fluorochrome-staining test), (2) sperm motility, (3) sperm morphology and (4) agglutination (using a computer assisted system) and (5) osmotic resistance (using the ORT). Parametric (analysis of variance for repeated measures) and non-parametric tests (Friedman test) were used as statistical analyses. Treatments with PGF2α concentrations higher than 12.5 mg/100 ml were cytotoxic while the others did not damage boar spermatozoa. Thus, the other treatments may be used to produce profitable effects without adverse effects. Moreover, the addition of PGF2α at 5 mg/100 ml to sperm diluted in BTS may maintain sperm viability and motility better after 6 days of cooling, because significant differences were observed (P < 0.05) compared with control at the same time Reconeixement-NoComercial-SenseObraDerivada 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/openAccess Senglar -- Espermatozoides Wild boar -- Spermatozoa Boar spermatozoa and prostaglandin F2alpha. Quality of boar sperm after addition of prostaglandin F2alpha to the short term extender over cooling time info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion peer-reviewed