2026-04-13T02:42:38Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/258722024-12-17T07:27:51Zcom_2072_452955com_2072_2054col_2072_453069

00925njm 22002777a 4500 dc Price, Daniel R.G. author Steele, Philip author Frew, David author McLean, Kevin author Androscuk, Dorota author Geldhof, Peter author Borloo, Jimmy author Palarea Albaladejo, Javier author Nisbet, Alasdair J. author McNeilly, Tom N. author 2024-06-01 Previous vaccination trials have demonstrated that thiol proteins affinity purified from Ostertagia ostertagi excretory-secretory products (O. ostertagi ES-thiol) are protective against homologous challenge. Here we have shown that protection induced by this vaccine was consistent across four independent vaccine-challenge experiments. Protection is associated with reduced cumulative faecal egg counts across the duration of the trials, relative to control animals. To better understand the diversity of antigens in O. ostertagi ES-thiol we used high-resolution shotgun proteomics to identify 490 unique proteins in the vaccine preparation. The most numerous ES-thiol proteins, with 91 proteins identified, belong to the sperm-coating protein/Tpx/antigen 5/pathogenesis-related protein 1 (SCP/TAPS) family. This family includes previously identified O. ostertagi vaccine antigens O. ostertagi ASP-1 and ASP-2. The ES-thiol fraction also has numerous proteinases, representing three distinct classes, including: metallo-; aspartyl- and cysteine proteinases. In terms of number of family members, the M12 astacin-like metalloproteinases, with 33 proteins, are the most abundant proteinase family in O. ostertagi ES-thiol. The O. ostertagi ES-thiol proteome provides a comprehensive database of proteins present in this vaccine preparation and will guide future vaccine antigen discovery projects http://hdl.handle.net/10256/25872 Parasitologia veterinària Veterinary parasitology Vacunes veterinàries Veterinary vaccines Nematodes Nematoda Characterisation of protective vaccine antigens from the thiol-containing components of excretory/secretory material of Ostertagia ostertagi