2026-04-13T04:55:16Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/243212024-06-18T13:38:51Zcom_2072_452955com_2072_2054col_2072_452958

00925njm 22002777a 4500 dc Puig Parnau, Irene author Segura i Torres, Pilar author García-Brito, Soleil author Huguet i Blanco, Gemma author Faghihi, Nastaran author Gubern Mérida, M. Carme author Kádár García, Elisabeth author 2020-03-26 Deep brain stimulation (DBS) of reward system brain areas, such as the medial forebrain bundle (MFB), by means of intracranial self-stimulation (ICSS), facilitates learning and memory in rodents. MFB-ICSS has been found capable of modifying different plasticity-related proteins, but its underlying molecular mechanisms require further elucidation. MicroRNAs (miRNAs) and the longevity-associated SIRT1 protein have emerged as important regulatory molecules implicated in neural plasticity. Thus, we aimed to analyze the effects of MFB-ICSS on miRNAs expression and SIRT1 protein levels in hippocampal subfields and serum. We used OpenArray to select miRNA candidates differentially expressed in the dentate gyrus (DG) of ICSS-treated (3 sessions, 45′ session/day) and sham rats. We further analyzed the expression of these miRNAs, together with candidates selected after bibliographic screening (miR-132-3p, miR-134-5p, miR-146a-5p, miR-181c-5p) in DG, CA1, and CA3, as well as in serum, by qRT-PCR.We also assessed tissue and serum SIRT1 protein levels byWestern Blot and ELISA, respectively. Expression of miR-132-3p, miR-181c-5p, miR-495-3p, and SIRT1 protein was upregulated in DG of ICSS rats (P < 0.05). None of the analyzed molecules was regulated in CA3, while miR-132-3p was also increased in CA1 (P = 0.011) and serum (P = 0.048). This work shows for the first time that a DBS procedure, specifically MFB-ICSS, modulates the levels of plasticity-related miRNAs and SIRT1 in specific hippocampal subfields. The mechanistic role of these molecules could be key to the improvement of memory by MFB-ICSS. Moreover, regarding the proposed clinical applicability of DBS, serum miR-132 is suggested as a potential treatment biomarker http://hdl.handle.net/10256/24321 Cervell -- Malalties -- Tractament Brain -- Diseases -- Treatment Cervell -- Estimulació Cervell -- Estimulació Brain stimulation Neurobiologia Neurobiology Intracranial self-stimulation modulates levels of SIRT1 protein and neural plasticity-related microRNAs