2026-04-03T21:12:07Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/243212024-06-18T13:38:51Zcom_2072_452955com_2072_2054col_2072_452958

2024-06-18T13:38:51Z urn:hdl:10256/24321 Intracranial self-stimulation modulates levels of SIRT1 protein and neural plasticity-related microRNAs Puig Parnau, Irene Segura i Torres, Pilar García-Brito, Soleil Huguet i Blanco, Gemma Faghihi, Nastaran Gubern Mérida, M. Carme Kádár García, Elisabeth Cervell -- Malalties -- Tractament Brain -- Diseases -- Treatment Cervell -- Estimulació Cervell -- Estimulació Brain stimulation Neurobiologia Neurobiology Deep brain stimulation (DBS) of reward system brain areas, such as the medial forebrain bundle (MFB), by means of intracranial self-stimulation (ICSS), facilitates learning and memory in rodents. MFB-ICSS has been found capable of modifying different plasticity-related proteins, but its underlying molecular mechanisms require further elucidation. MicroRNAs (miRNAs) and the longevity-associated SIRT1 protein have emerged as important regulatory molecules implicated in neural plasticity. Thus, we aimed to analyze the effects of MFB-ICSS on miRNAs expression and SIRT1 protein levels in hippocampal subfields and serum. We used OpenArray to select miRNA candidates differentially expressed in the dentate gyrus (DG) of ICSS-treated (3 sessions, 45′ session/day) and sham rats. We further analyzed the expression of these miRNAs, together with candidates selected after bibliographic screening (miR-132-3p, miR-134-5p, miR-146a-5p, miR-181c-5p) in DG, CA1, and CA3, as well as in serum, by qRT-PCR.We also assessed tissue and serum SIRT1 protein levels byWestern Blot and ELISA, respectively. Expression of miR-132-3p, miR-181c-5p, miR-495-3p, and SIRT1 protein was upregulated in DG of ICSS rats (P < 0.05). None of the analyzed molecules was regulated in CA3, while miR-132-3p was also increased in CA1 (P = 0.011) and serum (P = 0.048). This work shows for the first time that a DBS procedure, specifically MFB-ICSS, modulates the levels of plasticity-related miRNAs and SIRT1 in specific hippocampal subfields. The mechanistic role of these molecules could be key to the improvement of memory by MFB-ICSS. Moreover, regarding the proposed clinical applicability of DBS, serum miR-132 is suggested as a potential treatment biomarker 2024-06-18T13:38:51Z 2024-06-18T13:38:51Z 2020-03-26 info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion peer-reviewed http://hdl.handle.net/10256/24321 info:eu-repo/semantics/altIdentifier/doi/10.1007/s12035-020-01901-w info:eu-repo/semantics/altIdentifier/issn/0893-7648 info:eu-repo/semantics/altIdentifier/eissn/1559-1182 Tots els drets reservats info:eu-repo/semantics/openAccess Springer © Molecular Neurobiology, 2020, vol. 57, núm. 6, p. 2551-2562 Articles publicats (D-B) Puig-Parnau, Irene Segura i Torres, Pilar García-Brito, Soleil Huguet i Blanco, Gemma Faghihi, Nastaran Gubern Mérida, M. Carme Kádár García, Elisabeth 2020 Intracranial self-stimulation modulates levels of SIRT1 protein and neural plasticity-related microRNAs Molecular Neurobiology 57 6 2551 2562