2026-04-17T10:35:57Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/229222024-06-18T13:38:34Zcom_2072_452955com_2072_2054col_2072_452958

Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model Mateo Otero, Yentel Madrid-Gambín, Francisco Llavanera, Marc Gomez-Gomez, Alex Haro, Noemí Pozo, Oscar J. Yeste Oliveras, Marc Semen -- Fisiologia Semen -- Physiology Fecundació in vitro Fertilization in vitro Porcs -- Espermatozoides -- Investigació Swine -- Spermatozoa -- Research Whether basal metabolic activity in sperm has any influence on their fertilising capacity has not been explored. Using the pig as a model, the present study investigated the relationship of energetic metabolism with sperm quality and function (assessed through computer-assisted sperm analysis and flow cytometry), and fertility (in vitro fertilisation (IVF) outcomes). In semen samples from 16 boars, levels of metabolites related to glycolysis, ketogenesis and Krebs cycle were determined through a targeted metabolomics approach using liquid chromatography-tandem mass spectrometry. High-quality sperm are associated to greater levels of glycolysis-derived metabolites, and oocyte fertilisation and embryo development are conditioned by the sperm metabolic status. Interestingly, glycolysis appears to be the preferred catabolic pathway of the sperm giving rise to greater percentages of embryos at day 6. In conclusion, this study shows that the basal metabolic activity of sperm influences their function, even beyond fertilisation This study was funded by the Ministry of Science and Innovation, Spain (AGL2017-88329-R, FJC2018-035791-I, FPU18/00666 and PID2020-113320RBI00), the Regional Government of Catalonia, Spain (2017-SGR-1229 and 2020-FI-B00412), and the Catalan Institution for Research and Advanced Studies (ICREA) 2024-06-18T13:38:34Z 2024-06-18T13:38:34Z 2024-06-18T13:38:34Z 2023-03-30 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed http://hdl.handle.net/10256/22922 info:eu-repo/semantics/altIdentifier/doi/10.1038/s42003-023-04715-3 info:eu-repo/semantics/altIdentifier/eissn/2399-3642 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R/ES/MEJORA DEL RENDIMIENTO REPRODUCTIVO DEL SEMEN REFRIGERADO Y CONGELADO/DESCONGELADO DE PORCINO Y BOVINO MEDIANTE EL USO DE LA FOTOESTIMULACION/ Reconeixement 4.0 Internacional http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess Communications Biology, 2023, vol. 6, art.num. 344, p. 1-9 Articles publicats (D-B) Mateo Otero, Yentel Madrid-Gambín, Francisco Llavanera, Marc Gomez-Gomez, Alex Haro, Noemí Pozo, Oscar J. Yeste Oliveras, Marc 2023 Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model Communications Biology 6 Art.num. 344 1 9