2026-04-13T05:50:42Zhttps://recercat.cat/oai/requestoai:recercat.cat:10256/229222024-06-18T13:38:34Zcom_2072_452955com_2072_2054col_2072_452958
RECERCAT
author
Mateo Otero, Yentel
author
Madrid-Gambín, Francisco
author
Llavanera, Marc
author
Gomez-Gomez, Alex
author
Haro, Noemí
author
Pozo, Oscar J.
author
Yeste Oliveras, Marc
2024-06-18T13:38:34Z
2024-06-18T13:38:34Z
2023-03-30
http://hdl.handle.net/10256/22922
Whether basal metabolic activity in sperm has any influence on their fertilising capacity has not been explored. Using the pig as a model, the present study investigated the relationship of energetic metabolism with sperm quality and function (assessed through computer-assisted sperm analysis and flow cytometry), and fertility (in vitro fertilisation (IVF) outcomes). In semen samples from 16 boars, levels of metabolites related to glycolysis, ketogenesis and Krebs cycle were determined through a targeted metabolomics approach using liquid chromatography-tandem mass spectrometry. High-quality sperm are associated to greater levels of glycolysis-derived metabolites, and oocyte fertilisation and embryo development are conditioned by the sperm metabolic status. Interestingly, glycolysis appears to be the preferred catabolic pathway of the sperm giving rise to greater percentages of embryos at day 6. In conclusion, this study shows that the basal metabolic activity of sperm influences their function, even beyond fertilisationThis study was funded by the Ministry of Science and Innovation,
Spain (AGL2017-88329-R, FJC2018-035791-I, FPU18/00666 and PID2020-113320RBI00), the Regional Government of Catalonia, Spain (2017-SGR-1229 and 2020-FI-B00412), and the Catalan Institution for Research and Advanced Studies (ICREA)
Reconeixement 4.0 Internacional http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess
Semen -- Fisiologia
Semen -- Physiology
Fecundació in vitro
Fertilization in vitro
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed