2026-04-17T06:23:07Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/186782024-06-18T13:37:10Zcom_2072_452955com_2072_2054col_2072_452958

Single Layer Centrifugation improves the quality of fresh donkey semen and modifies their sperm ability to interact with polymorphonuclear neutrophils Papas, Marion Catalán, Jaime Recuero, Sandra Morrell, Jane Margaret Yeste Oliveras, Marc Miró, Jordi Espermatozoides Ases -- Espermatozoides -- Investigació Spermatozoa Donkeys -- Spermatozoa -- Research Ases -- Espermatozoides -- Anàlisi Donkeys -- Spermatozoa -- Analysis This study sought to determine whether single layer centrifugation (SLC) of fresh donkey semen with Equicoll has any impact on sperm quality parameters and on the modulation of endometrial reaction following semen deposition using an in vitro model. Seventeen ejaculates from five jackasses were obtained using an artificial vagina and diluted in a skim-milk extender. Samples were either selected through SLC (Equicoll) or non-treated (control). Two experiments were performed. The first one consisted of incubating selected or non-selected spermatozoa at 38 °C for 180 min. Integrity and lipid disorder of sperm plasma membrane, mitochondrial membrane potential, and intracellular levels of calcium and reactive oxygen species were evaluated at 0, 60, 120, and 180 min. In the second experiment, polymorphonuclear neutrophils (PMN) isolated from jennies blood were mixed with selected and unselected spermatozoa. Interaction between spermatozoa and PMN was evaluated after 0, 60, 120, and 180 min of co-incubation at 38 °C. SLC-selection increased the proportions of spermatozoa with an intact plasma membrane and low lipid disorder, of spermatozoa with high mitochondrial membrane potential and with high calcium levels, and of progressively motile spermatozoa. In addition, selection through SLC augmented the proportion of phagocytosed spermatozoa, which supported the modulating role of seminal plasma proteins on sperm-PMN interaction. In conclusion, SLC of fresh donkey semen increases the proportions of functionally intact and motile spermatozoa, and appears to remove the seminal plasma proteins that inhibit sperm-PMN binding This research was supported by the Ministry of Science, Innovation and Universities, Spain (Grants: RYC-2014-15581 and AGL2017-88329-R), and the Regional Government of Catalonia (2017-SGR-1229). J.C. was funded by the National Agency for Research and Development (ANID), Ministry of Education, Chile (Scheme: Becas Chile Doctorado en el Extranjero, PFCHA; Grant: 2017/72180128) 2024-06-18T13:37:10Z 2024-06-18T13:37:10Z 2024-06-18T13:37:10Z 2020-11-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed http://hdl.handle.net/10256/18678 info:eu-repo/semantics/altIdentifier/doi/10.3390/ani10112128 info:eu-repo/semantics/altIdentifier/eissn/2076-2615 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R/ES/MEJORA DEL RENDIMIENTO REPRODUCTIVO DEL SEMEN REFRIGERADO Y CONGELADO/DESCONGELADO DE PORCINO Y BOVINO MEDIANTE EL USO DE LA FOTOESTIMULACION/ info:eu-repo/grantAgreement/MINECO//RYC-2014-15581/ES/RYC-2014-15581/ Reconeixement 4.0 Internacional http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess Animals, 2020, vol. 10, núm. 11, p. 2128 Articles publicats (D-B) Papas, Marion Catalán, Jaime Recuero, Sandra Morrell, Jane Margaret Yeste Oliveras, Marc Miró, Jordi 2020 Single Layer Centrifugation improves the quality of fresh donkey semen and modifies their sperm ability to interact with polymorphonuclear neutrophils Animals 10 11 2128