2026-04-17T03:05:38Zhttps://recercat.cat/oai/request
oai:recercat.cat:10256/185512024-06-18T13:37:08Zcom_2072_452955com_2072_2054col_2072_452958
RECERCAT author García Martínez, Tania author Vendrell i Flotats, Meritxell author Martínez Rodero, Iris author Ordóñez León, Erika Alina author Álvarez Rodríguez, Manuel author López Béjar, Manel author Yeste Oliveras, Marc author Mogas Amorós, Teresa 2024-06-18T13:37:08Z 2024-06-18T13:37:08Z 2020-10-13 http://hdl.handle.net/10256/18551 This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) to the in vitro maturation (IVM) medium would improve the resilience of bovine oocytes to withstand vitrification. The effects of GSH-OEt on spindle morphology, levels of reactive oxygen species (ROS), mitochondrial activity and distribution, and embryo developmental potential were assessed together with the expression of genes with a role in apoptosis (BAX, BCL2), oxidative-stress pathways (GPX1, SOD1), water channels (AQP3), implantation (IFN-τ) and gap junctions (CX43) in oocytes and their derived blastocysts. Vitrification gave rise to abnormal spindle microtubule configurations and elevated ROS levels. Supplementation of IVM medium with GSH-OEt before vitrification preserved mitochondrial distribution pattern and diminished both cytoplasmic and mitochondrial ROS contents and percentages of embryos developing beyond the 8-cell stage were similar to those recorded in fresh non-vitrified oocytes. Although not significantly different from control vitrified oocytes, vitrified oocytes after GSH-OEt treatment gave rise to similar day 8-blastocyst and hatching rates to fresh non-vitrified oocytes. No effects of GSH-OEt supplementation were noted on the targeted gene expression of oocytes and derived blastocysts, with the exception of GPX1, AQP3 and CX43 in derived blastocysts. The addition of GSH-OEt to the IVM medium before vitrification may be beneficial for embryo development presumably as the consequence of additional anti-oxidant protection during IVM Reconeixement 4.0 Internacional http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess Bestiar boví -- Recursos germoplasmàtics -- Crioconservació Cattle -- Germplasm resources -- Cryopreservation Bestiar boví -- Fecunditat Cattle -- Fertility Estrès oxidatiu Oxidative stress Glutathione ethyl ester protects in vitro-maturing bovine oocytes against oxidative stress induced by subsequent vitrification/warming info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed