2026-04-14T02:50:45Zhttps://recercat.cat/oai/requestoai:recercat.cat:10256/184172024-06-18T13:37:04Zcom_2072_452955com_2072_2054col_2072_452958
RECERCAT
author
Vilanova Perez, Teresa
author
Jones, Celine
author
Balint, Stefan
author
Dragovic, Rebecca
author
Dustin, Michael L.
author
Yeste Oliveras, Marc
author
Coward, Kevin
2024-06-18T13:37:04Z
2024-06-18T13:37:04Z
2020-08-14
http://hdl.handle.net/10256/18417
Aims: To investigate exosomes as a non-invasive delivery tool for mammalian sperm. Materials & Methods: Exosomes were isolated from HEK293T cells and co-incubated with boar sperm in vitro. Results: Internalized exosomes were detected within 10 minutes of co-incubation. Computer-assisted sperm analysis and flow cytometry demonstrated that even after 5-hours of exposure to exosomes, there were no significant deleterious effects with regards to sperm motility, viability, membrane integrity and mitochondrial membrane potential (P>0.05), thus indicating that exosomes did not interfere with basic sperm function. Conclusion: HEK293T-derived exosomes interacted with boar sperm without affecting sperm function. Exosomes represent a versatile and promising research tool for studying sperm biology and provide new options for the diagnosis and treatment of male infertilityThe authors acknowledge generous support from the Ministry of Science, Innovation and Universities, Spain (Grants: RYC-2014-15581 and AGL2016-81890-REDT), and the Regional Government of Catalonia, Spain (Grant: 2017-SGR-1229), the Nuffield
Department of Women’s and Reproductive Health, University of Oxford, UK, the Wellcome Trust (100262Z/12/Z), Kennedy Trust for Rheumatology Research for Imaging facilities, and European Commission (ERC-2014-AdG 670930)
Reconeixement 4.0 Internacional http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess
Porcs -- Espermatozoides -- Investigació
Swine -- Spermatozoa -- Research
Mitocondris
Mitochondria
Exosomes derived from HEK293T cells interact in an efficient and non-invasive manner with mammalian sperm in vitro
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed