2026-04-17T11:29:28Zhttps://recercat.cat/oai/requestoai:recercat.cat:10256/182592024-06-18T12:40:54Zcom_2072_452955com_2072_2054col_2072_453079
RECERCAT
author
Santamaria Martos, Fernando
author
Benítez, Iván
author
Zapater, Andrea
author
Girón, Cristina
author
Pinilla, Lucía
author
Fernández-Real Lemos, José Manuel
author
Barbé, Ferran
author
Ortega Delgado, Francisco José
author
Sánchez de la Torre, Manuel
2024-06-18T12:40:54Z
2024-06-18T12:40:54Z
2019-03-13
http://hdl.handle.net/10256/18259
microRNAs (miRNAs) are non-coding RNAs highly relevant as biomarkers for disease. A seminal study that explored the role of miRNAs in obstructive sleep apnea syndrome (OSA) demonstrated their usefulness in clinical management. Nevertheless, the miRNAs that may act as endogenous controls (ECs) have not yet been established. The identification of ECs would contribute to the standardization of these biomarkers in OSA. The objective of the study is to identify miRNAs that can be used as ECs in OSA. We evaluated 100 patients divided into two different cohorts: a learning cohort of 10 non-OSA and 30 OSA patients, and a validation cohort (20 non-OSA and 40 OSA patients). In the learning cohort, a profile of 188 miRNAs was determined in plasma by TaqMan Low Density Array. The best EC candidates were identified by mean center+SD normalization and concordance correlation restricted normalization. The results were validated using NormFinder and geNorm to assess the stability of those ECs. Eight miRNAs were identified as EC candidates. The combination miRNA-106a/miRNA-186 was identified as the most stable among all candidates. We identified a set of ECs to be used in the determination of circulating miRNA in OSA that may contribute to the homogeneity of resultsThe project is supported by PI 14/01266 and PI 18/00449 from the Instituto de Salud Carlos III (ISCIII), Fondo Europeo de Desarrollo Regional (FEDER), Sociedad Española de Neumología y Cirugía Torácica (SEPAR) and Societat Catalana de Pneumologia (SOCAP). The work is supported by IRBLleida Biobank (B.0000682) and PLATAFORMA BIOBANCOS PT17/0015/0027”. We thank all the investigators of Sleep Unit of Santa Maria Hospital. F.S.-M. is the recipient of a predoctoral fellowship from “AGAUR-University of Lleida” and Convocatòria d’Ajuts 2018 de Promoció a la Recerca en Salut
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ info:eu-repo/semantics/openAccess
Síndromes d'apnea del son
Sleep apnea syndromes
MicroARN
MicroRNA
Marcadors genètics
Genetic markers
Identification and validation of circulating miRNAs as endogenous controls in obstructive sleep apnea
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed