2026-04-05T10:39:21Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/155062024-06-18T13:36:08Zcom_2072_452955com_2072_2054col_2072_452958

An Optimized high quality male DNA extraction from spermatophores in open thelycum shrimp species Planella Albi, Laia Heras Mena, Sandra Vera Rodríguez, Manuel García Marín, José Luis Roldán Borassi, María Inés ADN DNA Genotip Génotype Gambes -- Genètica Shrimps -- Genetics The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and quality. Several genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp species. However, all current protocols require invasive handling methods with males for DNA isolation. Using Aristeus antennatus as a model we tested a reliable non‐invasive differential DNA extraction method to male DNA isolation from spermatophores attached to female thelycum. The present protocol provides high quality and quantity DNA for polymerase chain reaction amplification and male genotyping. This new approach could be useful to experimental shrimp culture to select sires with relevant genetic patterns for selective breeding programs. More importantly, it can be applied to identify the mating pairs and male structure in wild populations of species as A. antennatus, where males are often difficult to capture. Our method could be also valuable for biological studies on other spermatophore‐using species, such as myriapods, arachnids and insect This work was supported by a grant from Spanish Ministerio de Economía y Competitividad (CTM2014-54648-C2-2-R) to MIR. LP benefited from predoctoral fellowship from the Universitat de Girona (BR2014) 2024-06-18T13:36:08Z 2024-06-18T13:36:08Z info:eu-repo/date/embargoEnd/2026-01-01 2017-09-01 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer-reviewed http://hdl.handle.net/10256/15506 info:eu-repo/semantics/altIdentifier/doi/10.1111/1749-4877.12250 info:eu-repo/semantics/altIdentifier/issn/1749-4869 info:eu-repo/semantics/altIdentifier/eissn/1749-4877 info:eu-repo/grantAgreement/MINECO//CTM2014-54648-C2-2-R/ES/CONECTIVIDAD POBLACIONAL EN LA GAMBA ROSADA, ARISTEUS ANTENNATUS, EN EL MEDITERRANEO NOROCCIDENTAL ENTRE EL GOLFO DE LEON Y EL GOLFO DE VALENCIA.-FLUJO GENICO./ Tots els drets reservats info:eu-repo/semantics/embargoedAccess Wiley © Integratize zoology, 2017, vol. 12, núm. 5, p. 421-427 Articles publicats (D-B)