2026-04-17T05:37:19Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/150532024-06-14T08:52:12Zcom_2072_452955com_2072_2054col_2072_453061

00925njm 22002777a 4500 dc Rabionet Díaz, Marc author Puig i Miquel, Teresa author Ciurana, Quim de author 2017 Only a small cell population of a breast tumour presents stem cell characteristics, thus so-called breast cancer stem cells (BCSCs). BCSCs are tumour-initiating cells and chemoresistant and they can grow in non-adherent conditions as mammospheres. Study of BCSCs is a challenge due to their low representation and the inability to propagate them without inducing differentiation. Previous studies have demonstrated that three-dimensional (3D) cell culture models such as scaffolds enhance the BCSCs population. In this project, scaffolds were fabricated by electrospinning technology. Specific values of voltage and flow rate were fixed to electrospun 7.5 and 15% poly(ɛ-caprolactone) (PCL) solutions according to process stability. Both scaffolds were seeded with MCF-7 breast cancer cells and 7.5% meshes displayed lower cell proliferation compared with 15% scaffolds. Cells cultured in both scaffolds presented a significant Mammosphere Forming Index (MFI) increase, thus indicating a BCSCs enrichment. Results show that three-dimensional cell culture with electrospun 15% PCL scaffolds could be useful to expand BCSCs population facilitating the future development of new therapeutic strategies against this tumour subpopulation This work was funded partially by Spanish grants from Fundación Ramón Areces, Instituto de Salud Carlos III (PI1400329) and by European Regional Development Fund (FEDER) and Ministerio de Economía y Competitividad (DPI2013-45201-P) and the support of the Catalonian government (2014SGR00868) http://hdl.handle.net/10256/15053 Mama -- Càncer Breast -- Cancer Materials nanoestructurats Nanostructured materials Polímers en medicina Polymers in medicine Nanotecnologia Nanotechnology Electrospinning Parameters Selection to Manufacture Polycaprolactone Scaffolds for Three-dimensional Breast Cancer Cell Culture and Enrichment