2026-04-17T14:18:09Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/124632024-06-18T13:35:30Zcom_2072_452955com_2072_2054col_2072_452958

00925njm 22002777a 4500 dc Balmaña, Meritxell author Giménez, Estela author Puerta, Angel author Llop Escorihuela, Esther author Figueras, Joan author Fort Martorell, Esther author Sanz Nebot, María Victoria author Bolòs, Carme de author Rizzi, Andreas author Barrabés Vera, Sílvia author Frutos, Mercedes de author Peracaula Miró, Rosa author info:eu-repo/date/embargoEnd/2026-01-01 info:eu-repo/date/embargoEnd/2026-01-01 2016-01-30 Pancreatic cancer (PDAC) lacks reliable diagnostic biomarkers and the search for new biomarkers represents an important challenge. Previous results looking at a small cohort of patients showed an increase in α-1-acid glycoprotein (AGP) fucosylation in advanced PDAC using N-glycan sequencing. Here,we have analysed AGP glycoforms in a larger cohort using several analytical techniques includingmass spectrometry (MS), capillary zone electrophoresis (CZE) and enzyme-linked lectin assays (ELLAs) for determining AGP glycoforms which could be PDAC associated. AGP from 31 serum samples, including healthy controls (HC), chronic pancreatitis (ChrP) and PDAC patients, was purified by immunoaffinity chromatography. Stable isotope labelling of AGP released N-glycans and their analysis by zwitterionic hydrophilic interaction capillary liquid chromatography electrospray MS (μZIC-HILIC–ESI-MS) showed an increase in AGP fucosylated glycoforms in PDAC compared to ChrP and HC. By CZE-UV analysis, relative concentrations of some of the AGP isoforms were found significantly different compared to those in PDAC and HC. Finally, ELLAs using Aleuria aurantia lectin displayed a significant increase in AGP fucosylation, before and after AGP neuraminidase treatment, in advanced PDAC compared to ChrP and HC, respectively. Altogether, these results indicate that α1-3 fucosylated glycoforms of AGP are increased in PDAC and could be potentially regarded as a PDAC biomarker This work was supported by the Government of Catalonia (grant 2014 SGR 229), the Spanish Ministry of Science and Innovation (grant BIO 2010-16922, awarded to R. P) and the Spanish Ministry of Economy and Competitiveness (grant CTQ2013-43236, awarded to M.F. and grant CTQ2011-27130, awarded to V. S-N) http://hdl.handle.net/10256/12463 Pàncrees -- Càncer Pancreas -- Cancer Marcadors tumorals Tumor markers Increased α1-3 fucosylation of α-1-acid glycoprotein (AGP) in pancreatic cancer