2026-04-13T01:33:00Zhttps://recercat.cat/oai/request

oai:recercat.cat:10256/124552024-06-18T13:35:28Zcom_2072_452955com_2072_2054col_2072_452958

Using Massive Parallel Sequencing for the Development, Validation, and Application of Population Genetics Markers in the Invasive Bivalve Zebra Mussel (Dreissena polymorpha) Peñarrubia Lozano, Luis Sanz Ball-llosera, Núria Pla Zanuy, Carles Vidal i Fàbrega, Oriol Viñas de Puig, Jordi Genètica de poblacions Population genetics Musclos d’aigua dolça -- Genètica Freshwater mussels -- Genetics Animals invasors Invasions biològiques Introduced organisms Biological invasions The zebra mussel (Dreissena polymorpha, Pallas, 1771) is one of the most invasive species of freshwater bivalves, due to a combination of biological and anthropogenic factors. Once this species has been introduced to a new area, individuals form dense aggregations that are very difficult to remove, leading to many adverse socioeconomic and ecological consequences. In this study, we identified, tested, and validated a new set of polymorphic microsatellite loci (also known as SSRs, Single Sequence Repeats) using a Massive Parallel Sequencing (MPS) platform. After several pruning steps, 93 SSRs could potentially be amplified. Out of these SSRs, 14 were polymorphic, producing a polymorphic yield of 15.05%. These 14 polymorphic microsatellites were fully validated in a first approximation of the genetic population structure of D. polymorpha in the Iberian Peninsula. Based on this polymorphic yield, we propose a criterion for establishing the number of SSRs that require validation in similar species, depending on the final use of the markers. These results could be used to optimize MPS approaches in the development of microsatellites as genetic markers, which would reduce the cost of this process 2024-06-18T13:35:28Z 2024-06-18T13:35:28Z 2024-06-18T13:35:28Z 2015 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/10256/12455 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0120732 info:eu-repo/semantics/altIdentifier/eissn/1932-6203 Attribution 3.0 Spain http://creativecommons.org/licenses/by/3.0/es/ info:eu-repo/semantics/openAccess Public Library of Science (PLoS) PLoS One, 2015, vol. 10, núm. 3, p.e0120732 Articles publicats (D-B)